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Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
UseTagsHis6x and mGFPmut3ExpressionBacterialMutationPromoterT7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-239
Plasmid#74609PurposeExpresses Hook domain and first coiled coil of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72R)
Plasmid#62082PurposeExpression of human p53 (1-73) (72R) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72RPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-p52(1-398)
Plasmid#44733DepositorInsertp52 (NFKB2 Human)
UseTagsExpressionBacterialMutationAA 1-398PromoterT7Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 G399S
Plasmid#16158DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationG399SPromoterT7Available sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28b-sad1-C63A
Plasmid#90302Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationC63APromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-GST
Plasmid#51528PurposeExpress GST-tagged hAIM2 in bacteriaDepositorInsertAIM2 (AIM2 Human)
UseTagsGSTExpressionBacterialMutationPromoterlac operatorAvailable sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p40)
Plasmid#135940PurposeExpresses AUF1p40 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b Me31B-3Myc
Plasmid#113001PurposeFor protein expression and purification of Myc-tagged full-length Drosophila me31BDepositorInsertfull length me31B (me31B Fly)
UseTagsMycExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-MDC
Plasmid#108950Purposeheterologous expression of MDC of S. cerevisiae in E. coliDepositorInsertMevalonate decarboxylase (MDC)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-PMK
Plasmid#108949Purposeheterologous expression of PMK of E. faecalis in E. coliDepositorInsertPhosphomevalonate kinase (PMK)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-IDI2
Plasmid#108951Purposeheterologous expression of IDI2 of T. thermophilus in E. coliDepositorInsertIsopentenyldiphosphat isomerase 2 (IDI2)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28 mSmo-Cterm
Plasmid#58703Purposeused for anti-mouse Smoothened antibody production (for affinity purification)DepositorInsertSmo (Smo Mouse)
UseTags6X-HISExpressionBacterialMutationcontains C terminus (aa 543-793)PromoterAvailable sinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29b-Ava3858-3857
Plasmid#45744DepositorInsertAva3858-3857
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET20b-delta133HtrA2 S400D
Plasmid#16156DepositorInsertHtrA2 (HTRA2 Human)
UseTagsHisExpressionBacterialMutationS400DPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-AACT
Plasmid#108944Purposeheterologous expression of AACT of M. sedula in E. coliDepositorInsertAcetoacetyl-CoA synthase (AACT)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-T-protein
Plasmid#12652DepositorInsertT-protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-deltaPYD
Plasmid#51522PurposeExpress His6-tagged HIN domain of hAIM2 in bacteriaDepositorInsertAIM2 (AIM2 Human)
UseTagsHis6 and T7ExpressionBacterialMutationdeletion of pyrin domainPromoterlac operatorAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only