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Plasmid#182948PurposeTo express the rat TrkC fused in the N-terminus with mVenusDepositorInsertmVenus-TrkC (Ntrk3 Synthetic, Rat)
UseRetroviral and Synthetic BiologyTagsmVenusExpressionMammalianMutationPromoterMMLVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
RABEP1A-c019
Plasmid#175477PurposeProtein expression in insect cells. Entire CDS, M1-T862. Codon optimised.DepositorInsertRABEP1 (RABEP1 Human)
UseTagsTEV-TwinStrepExpressionInsectMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
C108m
Plasmid#121010PurposeMoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized with pdt3 tag
UseSynthetic BiologyTagspdt3 mfLon degradation tagExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C103m
Plasmid#121007PurposeMoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
SQ171fg/pCSacB
Plasmid#69344PurposeSQ171 fast growing strain supported by pCSacB plasmidDepositorInsertpCSacB
UseTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-mNeonGreen
Plasmid#124101PurposeExpression of the fluorescent protein mNeonGreen in mammalian cellsDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationCodon usage was optimized for Homo SapiensPromoterCAGAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECNUS2
Plasmid#184885PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS4
Plasmid#184887PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS3
Plasmid#184886PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS1
Plasmid#184884PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-TrkC-mCherry
Plasmid#182949PurposeTo express the rat TrkC fused in the C-terminus with mCherryDepositorInsertTRk-C-mCherry (Ntrk3 Synthetic, Rat)
UseRetroviral and Synthetic BiologyTagsmCherryExpressionMammalianMutationPromoterMMLVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
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RABGEF1A-c014
Plasmid#175478PurposeProtein expression in insect cells. Entire CDS, M1-G491. Codon optimised.DepositorInsertRABGEF1 (RABGEF1 Human)
UseTagsHis6-Zbasic-TEVExpressionInsectMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SQ171fg/pRibo-T
Plasmid#69343PurposeSQ171 fast growing strain supported by pRibo-T plasmidDepositorInsertRibo-T
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-IRES2-p75NTR-GFP
Plasmid#182947PurposeTo express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-TerminalDepositorInsertp75NTR-mGFP
UseTagsHA tag and mGFPExpressionMammalianMutationThe leader sequence is from preprotrypinPromoterCAGAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-DBNF-mCherry
Plasmid#187195PurposeTo express the mouse BDNF tagged with the FP mCherryDepositorInsertBDNF
UseLentiviralTagsfluorescent protein mCherryExpressionMammalianMutationThe codons have been optimized for better express…PromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pBMCL1
Plasmid#178203PurposeAn improved vector for BacMam systemDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-FXN
Plasmid#23620DepositorInsertFXN (FXN Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
Plasmid#51083PurposeCre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV RetrogradeInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterhEF1aAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only