Addgene

We narrowed to 20,410 results for: crispr

Showing: 2041 - 2060 of 20410 results

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pColE1_sgRNA_M1S_AmpR

Plasmid

#119153

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M2S_AmpR

Plasmid

#119154

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M1NS_AmpR

Plasmid

#119155

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_M4NS_AmpR

Plasmid

#119156

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_M7NS_AmpR

Plasmid

#119157

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_T1_AmpR

Plasmid

#119158

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_T2_AmpR

Plasmid

#119159

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_sgRNA_T3_AmpR

Plasmid

#119160

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS143

Plasmid

#122377

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRS159

Plasmid

#122378

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pX330-LMNA-gRNA1

Plasmid

#122507

Purpose

expresses WT spCas9 and a chimeric gRNA targeting human LMNA

Depositor

Graham Dellaire

Article

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

LMNA-gRNA1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet promoter

Availability

Academic Institutions and Nonprofits only

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sgOpti

Plasmid

#85681

Purpose

Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

Depositor

David Sabatini, Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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pX330-H11r1-2-DD-Cas9

Plasmid

#85978

Purpose

Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pVDM1001

Plasmid

#134660

Purpose

CRISPR delivery and repair template delivery vector

Depositor

Willem van Schaik

Article

de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

Insert

CRISPR guide RNA array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF331_1

Plasmid

#135739

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF331_2

Plasmid

#135740

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB21_1

Plasmid

#135741

Purpose

Encodes gRNA for 3' target of human ZBTB21

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB21 gRNA (ZBTB21 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB21_2

Plasmid

#135742

Purpose

Encodes gRNA for 3' target of human ZBTB21

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB21 gRNA (ZBTB21 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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PX330_MLLT10_iso1_1

Plasmid

#135745

Purpose

Encodes gRNA for 3' target of human MLLT10_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MLLT10_iso1 gRNA (MLLT10 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

Enlarge