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Plasmid#148271PurposeBacterial Expression of DmThor_50-83-R56EDepositorInsertDmThor_50-83-R56E (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0664
Plasmid#141655PurposeLentiviral vector for overexpressing the NKX1-2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNKX1-2 (NKX1-2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AA
Plasmid#148272PurposeBacterial Expression of DmThor_50-83-R56ER63EDepositorInsertDmThor_50-83-R56ER63E (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV Δ18
Plasmid#169465PurposeEncodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV Spike truncated to remove 18 amino acids (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor_50-83-YMVLtoAADD_AB
Plasmid#148376PurposeInsect Expression of DmThor_50-83-YMVLtoAADDDepositorInsertDmThor_50-83-YMVLtoAADD (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-V75DL79D_AB
Plasmid#148377PurposeInsect Expression of DmThor-V75DL79DDepositorInsertDmThor-V75DL79D (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-P66KP71Q-GB1_AD
Plasmid#148516PurposeBacterial Expression of DmThor_50-83-P66KP71QDepositorInsertDmThor_50-83-P66KP71Q (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmThor-S65DT70D_AB
Plasmid#148375PurposeInsect Expression of DmThor-S65DT70DDepositorInsertDmThor-S65DT70D (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-V75DL79D-GB1_AB
Plasmid#148379PurposeBacterial Expression of DmThor_50-83-V75DL79DDepositorInsertDmThor_50-83-V75DL79D (Thor Fly)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHMMa.PH1704
Plasmid#11452DepositorInsertintracellular protease
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc-LATS2
Plasmid#66852PurposeExpress Myc-tagged LATS2 in mammalian cellsDepositorInsertLATS2 (LATS2 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK-hACE2
Plasmid#161612PurposeExpression of human ACE2 in mammalian cellsDepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_crGE
Plasmid#172933PurposeCrowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR: pPGK mCherry-HP1A
Plasmid#174115PurposeExpression of mCherry-HP1ADepositorInsertmCherry-HP1A
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis
Plasmid#192835PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cellsDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags3xFLAG-PreScission cleavage site and 6xhisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML433
Plasmid#177190PurposeExpresses recombinant HyPro enzyme in E. coliDepositorInsertHyPro enzyme
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-HA3-STONIN1
Plasmid#177580PurposeExpresses Stonin1 in mammalian cellsDepositorInsertSTONIN1
UseTags3XHA and GFPExpressionMammalianMutationV334A- please see depositor commentsPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR: pPGK PYL1-sfGFP-HP1A
Plasmid#174132PurposeExpression of PYL1-sfGFP-HP1ADepositorInsertPYL1-sfGFP-HP1A
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-STONIN2
Plasmid#177885PurposeEspresses Stonin2 in mammalian cellsDepositorInsertstonin2 (STON2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only