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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Use

Tags

TREER

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-PuRRRE

Plasmid

#208323

Purpose

Bacterial expression of the His-tagged peptide PuRRRE.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

PuRRRE

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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NK621

Plasmid

#196405

Purpose

Strong expression of firefly luciferase codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b-clbI S178A

Plasmid

#114159

Purpose

Expresses ClbI S178A

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbI

Use

Tags

6xHis and 6xHis-Thrombin site

Expression

Bacterial

Mutation

Mutated S178 to Ala

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-C-A2-PCP

Plasmid

#114157

Purpose

Expresses ClbH-C-A2-PCP

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH

Promoter

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCas8

Plasmid

#82380

Purpose

dCas9 without a promoter in acsA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCas73

Plasmid

#82398

Purpose

Δccm:Km, used to delete ccm operon

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

ccm deletion

Use

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLG-deltaA

Plasmid

#33151

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Use

Tags

LacZ

Expression

Yeast

Mutation

The RP51A sequence was modified by digestion with…

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA E171Q HDEL

Plasmid

#32123

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

K108A

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA K108A HDEL

Plasmid

#32121

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

HDEL and Kar2

Expression

Yeast

Mutation

K108A

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
HIR WT

Plasmid

#24049

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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HIR K1030R

Plasmid

#24050

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

K1030R

Promoter

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pMXs-puro-EGFP-FAK

Plasmid

#38194

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

PTK2 protein tyrosine kinase 2 (Ptk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MCS-BirA(R118G)-HA

Plasmid

#36047

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA(R118G)-HA

Use

Tags

HA

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

Available since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEF-BOS-RIG-I-Myc

Plasmid

#215872

Purpose

RIG-I-Myc expression vector for overexpression

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

Insert

RIG-I (RIGI Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1

Plasmid

#38193

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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p-G4PID-HA

Plasmid

#209639

Purpose

Expression G4PID-HA in mammalian cells

Depositor

Zhou Xiang

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

G4PID (DHX36 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMP2463

Plasmid

#107774

Purpose

Expression of EGFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EGFP in pBBR1-mcs5

Use

Tags

pLacZ-EGFP

Expression

Bacterial

Mutation

Promoter

pLacZ

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-mVenus-dspB(E184Q)-6xHis

Plasmid

#176576

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Use

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd3

Plasmid

#42255

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 3 (Fzd3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only