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Plasmid#120054PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-iDepositorInsertmScarlet-i
UsePcr templateTagsmScarlet-iExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB161 - pL2_pSB90_2x35S::GUS::tMAS
Plasmid#123197Purposebinary plant vector for transient expression of GUS (with introns)DepositorInsert2x35S::GUS::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab7 WT
Plasmid#44824DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (E1564A)
Plasmid#41587DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationE1564APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIND-CLucZ
Plasmid#53224PurposeInducible expression of Cypridina luciferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 Flag FKHR H215R AAA mutant
Plasmid#13510DepositorInsertFKHR H215R AAA (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationH215R T24A S256A S319APromoterAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'Q-mito
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP aā¦PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
SGL40C-H1.EFS.RFP657
Plasmid#69148PurposeAdvanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-LRRC38-V5-His
Plasmid#127820PurposeExpression of human LRRC38 in mammalian cellsDepositorInsertLRRC38 (LRRC38 Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
SRS-MTBD(22/19), AB(monomer), wt
Plasmid#22380DepositorInsertSeryl tRNA synthetase (SRS) ā mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsHis and mycExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
NELFE-mOrange pHis-//
Plasmid#53303PurposeExpresses His-tagged NELF-E-mOrange fusion protein.DepositorInsertNELF-E protein (Nelf-E Fly)
UseTagsHis and mOrangeExpressionBacterialMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRetro-XT-PAK2-CFP(puro)-L107F
Plasmid#81180PurposeRetroviral vector for expression of kinase active PAK2 with a CFP fusionDepositorInsertp21 (RAC1) activated kinase 2 (PAK2 Human)
UseRetroviralTagsCFPExpressionMutationL107FPromoterP-tight TREAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMJA181
Plasmid#128539PurposeExpresses anti-Prunus dulcis scFv Ab+BirA tagDepositorInsertscFv Ab clone PD1F6 plus BirA tag
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
C1-F-tractin-mCitrine
Plasmid#155217PurposeMonitor global F-actin distributionDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-258 A50I mutation
Plasmid#46271DepositorInsertVcl 1-258 (aka VD1) A50I mutation (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-258 (VD1 domain) A50I mutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1-His6
Plasmid#69109PurposeFor bacterial expression of recombinant human GTSE1 tagged with MBP and His.DepositorInsertG2- and S-phase Expressed 1 (GTSE1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-151
Plasmid#118533PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1b2
Plasmid#13752DepositorInsertJnk1b2 (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 MKK7b1(K149A)
Plasmid#14674DepositorInsertMKK7b1(K149A) (Map2k7 Mouse)
UseTagsExpressionMammalianMutationLys-149AlaPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk1a1
Plasmid#15743DepositorInsertJnk1a1 (MAPK8 Human)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
L40C-CRISPR.EFS.PAC
Plasmid#89393PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter drivenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
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S12.2_Bridge + Target
Plasmid#127671PurposeAmpicillin resistant multiplexing backbone with CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-157
Plasmid#118539PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
hP14Gal4
Plasmid#84364PurposeGal4-PRDM14 funsion plasmid for luciferase recruitment assayDepositorInsertPRDM14 (PRDM14 Human)
UseTagsGal4 and HA tagExpressionMammalianMutationsilent mutations at coding silent 1068CtoT, 1629Tā¦PromoterCMV/TOAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-160
Plasmid#118540PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-MCS-P2A-iLIDcaax
Plasmid#173864PurposeMammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)DepositorInsertmVenus-NES-SspB-MCS-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PTEN C124A
Plasmid#50520PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertPTEN (PTEN Human)
UseTagsGFPExpressionMammalianMutationC124APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-GĪ²Ī³-BERKY1
Plasmid#158429PurposeBRET biosensor for detection of free GĪ²Ī³ in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK3ct
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-CAML
Plasmid#22284DepositorInsertcalcium-modulating ligand (CAMLG Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk2b2
Plasmid#13757DepositorInsertJnk2b2 (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-163
Plasmid#118542PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-R9-GFP-HiBit
Plasmid#170990PurposeFor bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-R9-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-133
Plasmid#118519PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1/GgVcl 1-851 A50I mutant
Plasmid#46269DepositorInsertVcl 1-851 A50I mutation (VCL Chicken)
UseTagsEGFPExpressionMammalianMutation1-851 (Vh) head domain, A50I mutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-coemiRFP670-PGK-Puro
Plasmid#186168PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertemiRFP670
UseMarmoset targetingTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (D1320A)
Plasmid#41586DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationD1320APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
UseTagsGFP with 2A protease cleavage site between H2B anā¦ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7b2
Plasmid#14623DepositorInsertMKK7b2 (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP1-10 mitochondrial matrix
Plasmid#172063PurposeUAS vector to express GFP1-10 in the mitochondrial matrixDepositorInsertGFP1-10 mitochondrial matrix
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-131
Plasmid#118517PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk2b1
Plasmid#13756DepositorInsertJnk2b1 (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI2(8-193)
Plasmid#60356PurposeExpress the RBD of human MSI2 in bacterial cells for protein purificationDepositorInserthuman MSI2 (8-193) (MSI2 Human)
UseTagsHis and TEV protease siteExpressionBacterialMutationcontains amino acids 8-193 of MSI2PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-tac- YFP
Plasmid#186570Purposep15A ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-07-mNeonGreen-R11a
Plasmid#179890PurposeDonor with homologous arms for Rab11a to knock in mNeonGreenDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(40nm)-Cys
Plasmid#91571PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(40nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Empty Anchor Control
Plasmid#127665PurposeEncodes for puromycin resistance expressed from the EF1a promoterDepositorInsertPuro
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RapR-FAK-YM-Y397F
Plasmid#25930DepositorInsertRapR-FAK (Ptk2 Mouse, Human)
UseTagsEGFPExpressionMammalianMutationThis pEGFP-RapR-FAK-YM-Y397F plasmid contains theā¦PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pet16b ALIX Bro1 (1-359)
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only