Addgene

We narrowed to 47,817 results for: ANG

Showing: 3061 - 3090 of 47817 results

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pU6-gRNA-TAZ

Plasmid

#86688

Purpose

Expresses a gRNA targeted to human TAZ

Depositor

William Pu

Article

Wang et al Nat Protoc. 2017 Jan;12(1):88-103. doi:

Insert

U6-gRNA-TAZ (TAFAZZIN Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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PLK3 1-334 T219D-pEGFP

Plasmid

#23268

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Use

Tags

pEGFP

Expression

Mammalian

Mutation

aa 1-334 T219D

Promoter

Available since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only
pDUAL-P41nmt1-Nup124-GFP-MTn

Plasmid

#154365

Purpose

Yeast expression of Nup124

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Nup124 (nup124 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

See depositor comments below

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_HOXD13-mcherry

Plasmid

#159793

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting HOXD13 gene (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Px458-Cas9n-Trp73-Exon4-3sgRNA

Plasmid

#88850

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73 (Trp73 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Px458-Cas9n-Trp73-Exon4-4sgRNA

Plasmid

#88851

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73 (Trp73 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Flag-ZDHHC22

Plasmid

#231670

Purpose

Expresses human ZDHHC22 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC22 (ZDHHC22 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC14

Plasmid

#231662

Purpose

Expresses human ZDHHC14 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC14 (ZDHHC14 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC13

Plasmid

#231661

Purpose

Expresses human ZDHHC13 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC13 (ZDHHC13 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC5

Plasmid

#231654

Purpose

Expresses human ZDHHC5 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC5 (ZDHHC5 Human)

Use

Tags

3×Flag Tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-UnaG-P2A-FusionRed

Plasmid

#191109

Purpose

Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

UnaG

Use

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pHD22Y/PfPRMT5 deletion

Plasmid

#203161

Purpose

for disruption of PfPRMT5 gene

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment

Use

Tags

3 myc tags and 3' UTR of P. berghei dihydrof…

Expression

Mammalian

Mutation

Promoter

No

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pnCas9-miniSdd3-PBE

Plasmid

#204857

Purpose

For cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TALE-R-JAK2-Ddd9-C

Plasmid

#204854

Purpose

For DdCBE editing using Ddd9 at JAK2 site in human cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

Ddd9-C

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TALE-L-JAK2-Ddd9-N

Plasmid

#204853

Purpose

For DdCBE editing using Ddd9 at JAK2 site in human cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

Ddd9-N

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shPP1C #2

Plasmid

#198763

Purpose

conditional knockdown of PP1C

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shPP1C #2 (PPP1CC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shPP1C #1

Plasmid

#198762

Purpose

conditional knockdown of PP1C

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shPP1C #1 (PPP1CC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-GPI

Plasmid

#177264

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via CD59 GPI) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

CD59 leader-FRISZ-GPI

Use

AAV

Tags

CD59 GPI and CD59 leader sequence

Expression

Mutation

Promoter

hSyn

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-TBG-Cre-sgArid1a

Plasmid

#192165

Purpose

AAV-TBG-Cre-sgArid1a

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-TBG-Cre-sgKmt2d

Plasmid

#192164

Purpose

AAV-TBG-Cre-sgKmt2d

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

Plasmid

#168251

Purpose

"label active Rac under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

Plasmid

#168254

Purpose

"label stable F-actin under Rac2 neutrophil-specific knockout background"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTW045

Plasmid

#185690

Purpose

T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

Depositor

Feng Zhang

Article

Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

Insert

Cas9, Drm1b gRNAs

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTW037

Plasmid

#185688

Purpose

T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

Depositor

Feng Zhang

Article

Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

Insert

Cas9, Drm1b gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FluoSTEP-AKAR (C/Y)

Plasmid

#181862

Purpose

FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (C-Y)

Use

Tags

CFP(1-10) and cpVenus(E127)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

Plasmid

#168252

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

PBD GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

Plasmid

#168253

Purpose

"label stable F-actin; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Utrophin-GFP

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

LyzC

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-DGCR8(+6)

Plasmid

#171541

Purpose

Encodes mutant human DGCR8 protein

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

DGCR8 (DGCR8 Human)

Use

Tags

Expression

Mammalian

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Promoter

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-acVHH (Y34W)-PB

Plasmid

#171072

Purpose

A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

Depositor

Yubin Zhou

Article

Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

Insert

anti-caffeine nanobody variant

Use

Tags

modified STIM1–PB tag

Expression

Mammalian

Mutation

Y34W

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-acVHH (Y34F)-PB

Plasmid

#171071

Purpose

A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

Depositor

Yubin Zhou

Article

Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

Insert

anti-caffeine nanobody variant

Use

Tags

modified STIM1–PB tag

Expression

Mammalian

Mutation

Y34F

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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