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Showing: 3061 - 3090 of 148365 results

  1. pJRH029

    Plasmid
    #171060
    Purpose
    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Insert
    Gag-spyCas9
    Use
    CRISPR and Lentiviral
    Tags
    2x SV40 NLS and 3x FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171060-plasmid-map-sequence-id-336494

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  10. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Peter Schultz
    Article
    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31190-plasmid-map-sequence-id-418765

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  15. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  19. pSNAP-tag (m) Vector

    Plasmid
    #101135
    Purpose
    Expression of protein of interest with SNAP-Tag in Mammalian cells
    Depositor
    Ana Egana, New England Biolabs
    Type
    Empty backbone
    Use
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101135-plasmid-map-sequence-id-194101

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  28. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61408-plasmid-map-sequence-id-333613

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  30. pLenti.PGK.LifeAct-Ruby.W

    Plasmid
    #51009
    Purpose
    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-mRFPruby
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51009-plasmid-map-sequence-id-339916

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Showing: 3061 - 3090 of 148365 results