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STK4A-c322
Plasmid#53630Purposebacterial expression plasmidDepositorInsertSTK4A (STK4 Human)
UseTagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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PF10_0086:MAC044-A01:C220746
Plasmid#183979PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertPF10_0086
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMCSG53_Nsp3_Y_3
Plasmid#167263PurposeBacterial expression of Nsp3 Y domain (residues 1844-1945)DepositorInsertNsp3 (residues 1844-1945) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCSG53_Nsp3_Y_2
Plasmid#167262PurposeBacterial expression of Nsp3 Y domain (residues 1619-1945)DepositorInsertNsp3 (residues 1619-1945) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCSG53_Nsp3_Ubl1
Plasmid#167257PurposeBacterial expression of Nsp3 ubiquitin-like domain (1-111)DepositorInsertNsp3 (residues 1-111) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCSG53_Nsp3_Y_1
Plasmid#167261PurposeBacterial expression of Nsp3 Y domain (residues 1584-1945)DepositorInsertNsp3 (residues 1584-1945) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCSG53_Nsp3_NAB_2
Plasmid#167260PurposeBacterial expression of Nsp3 Nucleic acid binding domain (residues 1089-1203)DepositorInsertNsp3 (residues 1089-1203) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCSG53_Nsp3_NAB_1
Plasmid#167259PurposeBacterial expression of Nsp3 Nucleic acid binding domain (residues 1050-1216)DepositorInsertNsp3 (residues 1050-1216) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pExp-TFSumo
Plasmid#112576PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TFSumo and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-Tsf
Plasmid#112578PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-Tsf and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only