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pRS426-TUB1-internal6xHis

Plasmid

#60390

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-Cas9

Plasmid

#166601

Purpose

Encodes Cre-dependent Cas9 under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

Cas9

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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3440 pcDNA3 Bid p22

Plasmid

#8774

Depositor

Stanley Korsmeyer

Article

Zha et al Science 2000 Dec 1;290(5497):1761-5.

Insert

Bid p22 wt (Bid Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK-kinase0

Plasmid

#59122

Purpose

FAK fret control sensor without FERM domain with no linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-miR-20-Control

Plasmid

#21169

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Insert

miR-20 Sensor (MIR20A Human)

Use

Tags

Expression

Mammalian

Mutation

Sensor construct insert has two miR-20 binding si…

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
myc-UVRAG S498A

Plasmid

#86737

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

Insert

human UVRAG (UVRAG Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S498A

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM-7Zf-TYR

Plasmid

#32786

Depositor

Ruth Halaban

Article

Ujvari et al J Biol Chem. 2001 Feb 23;276(8):5924-31.

Insert

Tyrosinase (TYR Human)

Use

In vitro transcription

Tags

Expression

Mutation

I181M

Promoter

T7

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL

Plasmid

#24242

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pMDC43-SEIPIN3

Plasmid

#96978

Purpose

Express GFP-tagged Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Use

Tags

GFP

Expression

Plant

Mutation

Promoter

35S

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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SEPT5 Mut(S327A)

Plasmid

#27274

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Use

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

S327A

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
Cdc42 T17N sp64T

Plasmid

#16672

Depositor

De-Li Shi

Article

Djiane et al Development. 2000 Jul . 127(14):3091-100

Insert

Cdc42 T17N (CDC42 Human)

Use

Xenopus expression

Tags

Expression

Mutation

T17N. Dominant negative.

Promoter

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
pSuper.Retro.Puro-WDR5 shRNA 4

Plasmid

#59977

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO/intron/FlagCter

Plasmid

#113548

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

Flag

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_PKA-Cα(K8,9A)-mPAGFP

Plasmid

#168493

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5 KSR1 S392A

Plasmid

#25976

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed serine 392 to alanine

Promoter

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
GFP Snail 4SA

Plasmid

#16227

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

4SA: S108A, S112A, S116A, S120A

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pCAGGS/Daxx myc S669A

Plasmid

#19282

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc S669A (Daxx Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
721-2µ-staHIS3

Plasmid

#40611

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-FERM0

Plasmid

#59125

Purpose

FAK fret control sensor without kinase domain with no linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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