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Plasmid#192834PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags6xhisExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(306-593)-6xhis
Plasmid#192837PurposeExpress codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags6xhisExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
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PB-NLS-ZapCV2
Plasmid#203664PurposeExpresses zinc sensor ZapCV2 in the nucleus of mammalian cellsDepositorInsertZapCV2 Zinc sensor
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-PCNA
Plasmid#105977Purposeentry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNADepositorInsertPCNA (PCNA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Ssh1 gRNA#1
Plasmid#163394PurposeCas9-mediated knockout of Ssh1 in mammalian cellsDepositorInsertssh1 (Ssh1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ssh1 gRNA#3
Plasmid#163396PurposeCas9-mediated knockout of Ssh1 in mammalian cellsDepositorInsertssh1 (Ssh1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pnEA-NpM-DmThor_32-83-GB1_AA
Plasmid#148267PurposeBacterial Expression of DmThor_32-83DepositorInsertDmThor_32-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1415
Plasmid#84828PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 intronsDepositorInsertC. elegans codon optimized GFP
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-R56ER63ER81E-GB1_AB
Plasmid#148378PurposeBacterial Expression of DmThor_50-83-R56ER63ER81EDepositorInsertDmThor_50-83-R56ER63ER81E (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-R63E-GB1_AA
Plasmid#148273PurposeBacterial Expression of DmThor_50-83-R63EDepositorInsertDmThor_50-83-R63E (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-YMVLtoAADD-GB1_AA
Plasmid#148274PurposeBacterial Expression of DmThor_50-83-YMVLtoAADDDepositorInsertDmThor_50-83-YMVLtoAADD (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_32-83-S65DT70D-GB1_AA
Plasmid#148268PurposeBacterial Expression of DmThor_32-83-S65DT70DDepositorInsertDmThor_32-83-S65DT70D (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_32-83-YMSTtoAADD-GB1_AA
Plasmid#148269PurposeBacterial Expression of DmThor_32-83-YMSTtoAADDDepositorInsertDmThor_32-83-YMSTtoAADD (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-R56AR63A-GB1_AA
Plasmid#148270PurposeBacterial Expression of DmThor_50-83-R56ER63ADepositorInsertDmThor_50-83-R56ER63A (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only