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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A

Plasmid

#215900

Purpose

Expression of TNRC6A-CIM1 domain with S1332A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1332A

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1346A

Plasmid

#215901

Purpose

Expression of TNRC6A-CIM1 domain with S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1346A

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A

Plasmid

#215903

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1616A/S1691A

Plasmid

#215904

Purpose

Expression of TNRC6A-silencing domain (SD) with S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1616A, S1691A

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-KIF13AWLDLE tail 361-1749

Plasmid

#224412

Purpose

Mammalian expression of KIF 13a WLDLE

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a WLDLE (Kif13a Mouse)

Use

Tags

FRB-3myc

Expression

Mammalian

Mutation

WLDLE

Promoter

Chicken Beta actin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

Plasmid

#224489

Purpose

Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Sun1-2xsfGFP-6xMYC-pA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS1 (JDW 1095)

Plasmid

#224510

Purpose

Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Myc-BioID2

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS2 (JDW 1209)

Plasmid

#224511

Purpose

A Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCS

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Myc-BioID2

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-KIF13AS1371A tail 361-1749

Plasmid

#224411

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Use

Tags

FRB-3myc

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-IgKappa-myc-dL5- 2XG4S-GPI

Plasmid

#153308

Purpose

Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)

Depositor

Marcel Bruchez

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

IgKappa-myc-dL5-2XG4S-GPI

Use

Tags

myc-dL5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/S170D/D338N

Plasmid

#213595

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/D338N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, D338N

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/S170D/Y280A

Plasmid

#213596

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/Y280A cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, Y280A

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D/D321N

Plasmid

#213598

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/D321N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D, D321N

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D/Y263A

Plasmid

#213599

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D/Y263A cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D, Y263A

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Plasmid

#202064

Purpose

Inducible AAV activator

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al bioRxiv. (PrePrint)

Insert

AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Use

AAV; Aav with reporter

Tags

Expression

Mammalian

Mutation

ITR deletion

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

Plasmid

#210021

Purpose

Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Synthetic, Human)

Use

Tags

MycTag, CTag

Expression

Mammalian

Mutation

Transferrin receptor transmembrane domain with Y2…

Promoter

CMV promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212740

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212739

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

Plasmid

#212738

Purpose

Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

HOIP(C885A)

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV

Plasmid

#212737

Purpose

Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge