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pDONR221-OSF-TRIM5-21R
Plasmid#79063PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertOSF-TRIM5-21R
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNEXdelta-ApAF
Plasmid#13334DepositorInsertAplysia activating factor
UseTagsExpressionMutationPromoterAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mTagBFP2-WPRE
Plasmid#193332PurposeExpresses mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173875PurposeMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173876PurposeMammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetro-XT-PAK2-CFP(puro)-WT
Plasmid#81176PurposeRetroviral vector for expression of wild-type PAK2 with a CFP fusionDepositorInsertp21 (RAC1) activated kinase 2 (PAK2 Human)
UseRetroviralTagsCFPExpressionMutationPromoterP-tight TREAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-2_PolyU(1)_Tornado-Corn
Plasmid#159487PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-2_PolyU(1)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MBD1
Plasmid#47057PurposeExpresses methyl-CpG binding domain protein 1DepositorInsertMethyl-CpG Binding Domain Protein 1 (MBD1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-161
Plasmid#118541PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcihygroflagsynhDAT
Plasmid#19990DepositorInsertDopamine transporter (SLC6A3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a-scrambled)
Plasmid#79671PurposeScrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNICE HA-mSYD1B
Plasmid#59362PurposeExpression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)DepositorInsertmSYD1B (Syde2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-157
Plasmid#118571PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-156
Plasmid#118538PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6015nes
Plasmid#108657PurposeA S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-S6K-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
3349 pSFFV-neo Bcl-2
Plasmid#8769DepositorInsertBcl-2 (BCL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6013x
Plasmid#108654PurposeAn HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertRaichu-EV
UseLentiviralTagsXFPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-rhesusTRIM5-FOS
Plasmid#79059PurposeExpress rhesus TRIM5 in mammalian cellsDepositorInsertrhesusTRIM5-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorUseTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-HA-GAAZFP-FokI-RR
Plasmid#18754DepositorInsertzinc finger nuclease
UseTagsExpressionBacterialMutationRNA expression vector for expressing the zinc fin…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-6017nes
Plasmid#108655PurposeA PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-PKA-EV
UseTagsXFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
S12.1_Empty_Bridge
Plasmid#127670PurposeAmpicillin resistant multiplexing backbone without CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk3a2
Plasmid#15748DepositorInsertJnk3a2 (MAPK10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRetro-XT-PAK2-CFP(puro)-T402A
Plasmid#81179PurposeRetroviral vector for expression of kinase inactive PAK2 with a CFP fusionDepositorInsertp21 (RAC1) activated kinase 2 (PAK2 Human)
UseRetroviralTagsCFPExpressionMutationT402APromoterP-tight TREAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P25
Plasmid#120036PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LIDDepositorInsertYFP-LID
UsePcr templateTagsYFP-LIDExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-hu FceRI beta
Plasmid#8366DepositorInserthuman Fc epsilon RI beta cDNA (MS4A2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
SLF091: Lenti-CaMKIIa-eTsChR-eGFP
Plasmid#112281PurposeFor lentiviral or direct expression of eTsChR in excitatory neuronsDepositorInserteTsChR
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUB-spHRed
Plasmid#84734PurposeExpresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stomaDepositorInsertpHRed
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBstSK+IRES-V5-IPpoI (mutant)
Plasmid#206322PurposeA plasmid that facilitates production of mRNA encoding V5 epitope tagged catalytically dead I-PpoIDepositorInsertIRES element-V5 epitope tag-mutated IPpoI open reading frame
UseMrna productionTagsV5-IPpoI fusion protein (mutant version)ExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RapR-FAK-YM-KD
Plasmid#25929DepositorInsertRapR-FAK (Ptk2 Mouse, Human)
UseTagsEGFPExpressionMammalianMutationThis pEGFP-RapR-FAK-YM-KD plasmid contains the FA…PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Tubulin-6
Plasmid#57974PurposeLocalization: Microtubules, Excitation: 549, Emission: 565DepositorInsertTubulin (TUBA1B Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro2 E208K/E328K
Plasmid#47900PurposeExpresses myc tagged Miro2 V208K/E328K mutantDepositorInsertMiro2 E208K/E328K (RHOT2 Human)
UseTagsmycExpressionMammalianMutationE208K/E328K, abolishes calcium bindingPromoterCMVAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-ZF5.3-GFP-HiBit
Plasmid#170992PurposeFor bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN-R2-mCsfGFP-L3
Plasmid#178602PurposeIt can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.DepositorInsertTandem fluorescent protein timers (tFTs)
UseEntry vector for gateway cloningTagsmCherry superfolder GFPExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐miniABEmax‐N2‐ E53ogRNA
Plasmid#187065PurposeGp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertminiABEmax-Gp41-1
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA
Plasmid#187066PurposeGp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertGp41-1-ABEmaxNGA
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Flag-TTGZFP-FokI-DD
Plasmid#18755DepositorInsertzinc finger nuclease
UseTagsExpressionBacterialMutationRNA expression vector for expressing the zinc fin…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SHP2 D61A
Plasmid#8330DepositorInsertSHP2 (PTPN11 Human)
UseRetroviralTagsExpressionMammalianMutationD61A (activated mutant)PromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (Y971A/Y972A)
Plasmid#41590DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationY971A/Y972APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA
Plasmid#158989PurposeVector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neuronsDepositorInsertdSaCas9-SID4X
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_WTTS
Plasmid#91244Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationD27NPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_WTTS
Plasmid#91229Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_G121VDHFR_WTTS
Plasmid#91233Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HOXA1
Plasmid#47054PurposeHomeobox A1DepositorInsertHomeobox A1 (HOXA1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro1 K427V
Plasmid#47892PurposeExpresses myc tagged Miro1 K427 constitutively active mutantDepositorInsertMiro1 K427V (RHOT1 Human)
UseTagsmycExpressionMammalianMutationK427V, constitutively active mutantPromoterCMVAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
UseTagsHis and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only
