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pHM6-alphasynuclein-WT

Plasmid

#40824

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Use

Tags

6x His and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

Plasmid

#185713

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed W403 and F753 to A

Promoter

T7

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-melanopsin-mCherry

Plasmid

#122630

Purpose

AAV-mediated expression of melanopsin-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Mederos et al Glia. 2019 May;67(5):915-934. doi: 10.10

Insert

melanopsin-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

GFAP104

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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Calnexin-HT8-mGold

Plasmid

#183989

Purpose

Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

Calnexin (CANX Human)

Use

Tags

HaloTag8 (C61S, C262S), mGold

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII-ShadowG

Plasmid

#167475

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2pACryGFP

Plasmid

#64022

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

Depositor

Peter Currie, Joachim Berger

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

GFP

Use

Gateway

Tags

Expression

Mutation

Promoter

alpha-Acrystallin promoter

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

Plasmid

#188902

Purpose

dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-KRAB(Kox1)-MeCP2

Use

Lentiviral

Tags

NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

Plasmid

#166611

Purpose

Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)

Plasmid

#160044

Purpose

For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

iaaH (iaaH )

Use

Synthetic Biology

Tags

3xNLS-Citrine

Expression

Mammalian

Mutation

Promoter

pEF1s

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHR FGFR1-CD3ε-FusionRed

Plasmid

#188632

Purpose

Human FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

FGFR1 (FGFR1 Human, Synthetic)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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