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Plasmid#25865DepositorInsertPRS driven enhanced green fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap-KO
Plasmid#196489PurposeTo KO Gfap in mouse astrocytesDepositorInsertgRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gfaABC1D-mCherry-CAAX
Plasmid#218189PurposeTo over express mCherry-CAAX under gfaABC1D promoter in astrocytesDepositorInsertmCherry-CAAX
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSLR
Plasmid#51500PurposeSingle component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.DepositorTypeEmpty backboneUsePlant t-dna plasmidTagsExpressionMutationPromoterAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
H2B-SNAPf-mTurquoise2
Plasmid#197494PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the nucleus.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNAPf-mTurquoise2
Plasmid#197495PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the whole cell.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-melHis10TEV
Plasmid#159428Purposeempty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sitesDepositorTypeEmpty backboneUseTagsmelittin signal sequence (removed in vivo by sign…ExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMyc0ElbLuc
Plasmid#53242Purposereporter plasmid without myc cDNA binding sitesDepositorInsertpMycOElbLuc (MYC Human)
UseTagsExpressionMutationPromoterAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
TOM20-SNAPf-mTurquoise2
Plasmid#197496PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the mitochondria.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP
Plasmid#51494PurposepLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmidDepositorInsertsGFP
us:NPTII repair template
UsePlant t-dna plasmidTagsNLS and flagExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mutLuc
Plasmid#106458Purposeluciferase reporter, CMV promoter (3 NFkB sites mutated)DepositorInsertluciferase reporter, CMV promoter (3 NFkB sites mutated)
UseLuciferaseTagsExpressionMammalianMutation3 NFkB sites mutatedPromoterAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBPcyto-TEV-BseRI
Plasmid#157739Purposeempty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + MBP (cytoplasmic expression; no signal se…ExpressionBacterialMutationPromoterT7Available sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
p35ST
Plasmid#51514PurposepMDC32 with T30 TALEN pair followed by the us:NPTII donor moleculeDepositorInsertsT30 TALEN pair
us:NPTII repair template
UsePlant expression t-dnaTagsNLS and flagExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
p35SZ.D
Plasmid#51512PurposepMDC32 with Zif268:FokI followed by us:NPTII repair template sequenceDepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant expression t-dnaTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pgfaABC1D-mCherry-CAAX
Plasmid#196488PurposeTo over express mCherry-CAAX under gfaABC1D promoter in astrocytesDepositorInsertmCherry-CAAX
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNAPf-mTurquoise2-KDEL
Plasmid#197498PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-SNAPf-mTurquoise2
Plasmid#197497PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in actin filaments.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMini-WT
Plasmid#179257Purposeexpresses GFP CDS-derived linear and circular RNA transcript. Flanking introns are devoid of NOVA2 binding sitesDepositorInsertcircGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMini-Both
Plasmid#179258Purposeexpresses GFP CDS-derived linear and circular RNA transcript. Flanking introns contain NOVA2 binding sitesDepositorInsertcircGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRVV700
Plasmid#119017PurposeDonor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertTP901-1 attP sites
UseCRISPR; Donor vector for creation of platforms fo…TagsExpressionMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only