Addgene

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP2-NS

Plasmid

#80502

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP2

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

TagBFP2 (no STOP)

Use

Multisite gateway middle entry clone

Tags

Expression

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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MSGV Rh Acceptor

Plasmid

#64271

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCoofy41

Plasmid

#55184

Purpose

Insect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

Expression

Bacterial

Mutation

Promoter

pPH (Polyhedrin promoter)

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-GFP no-pA

Plasmid

#80813

Purpose

GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

GFP

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:nlsGFP pA

Plasmid

#80821

Purpose

CMVmin-promoted nlsGFP with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-nlsGFP pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVmin

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pME nls-eos

Plasmid

#73791

Purpose

Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

Depositor

David Raible

Article

Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

Insert

nls-eos

Use

Middle entry clone

Tags

Expression

Mutation

Promoter

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::PuroR-bGHpA

Plasmid

#62440

Purpose

MXS_chaining vector with PGK::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCWX-R4-DEST-R2-PGR65

Plasmid

#45953

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

internal BsaI recognition site in 35S promoter re…

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFP no-pA

Plasmid

#80819

Purpose

CMVmin-promoted GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-GFP no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_MCS1

Plasmid

#62395

Purpose

MXS_chaining vector with MCS1

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF4

Plasmid

#122464

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 (SCAF4 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-GFPmem no-pA

Plasmid

#80817

Purpose

GFPmem without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFPcaax no pA p3E

Use

Tags

Expression

Mammalian

Mutation

Promoter

GFP

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pNH053

Plasmid

#42159

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mCherry-RT-mCherry

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::NeoR-bGHpA

Plasmid

#62438

Purpose

MXS_chaining vector with PGK::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-SARS-CoV-2 S1 RBD

Plasmid

#167013

Purpose

Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Tags

Expression

Mammalian

Mutation

No stop codon

Promoter

Available since

April 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pME-mKate2 no stop

Plasmid

#80806

Purpose

red fluorophore without stop codon

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-mKate2 no stop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCoofy42

Plasmid

#55190

Purpose

Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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