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pLenti-Arch-EEQ

Plasmid

#45188

Purpose

Arch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95N

Depositor

Mark Schnitzer

Article

Gong et al PLoS One. 2013 Jun 19;8(6):e66959.

Insert

enhanced Archaerhodopsin

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Mutation

D95Q, D106E

Promoter

CamKIIa

Available since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGECInano

Plasmid

#186188

Purpose

AAV vector packaging small FRET-based near-infrared genetically-encoded calcium indicator

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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Syn:eGFP-CaM-uTEV1Δ(220-242)

Plasmid

#135463

Purpose

Neuronal expression of uFLARE protease component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

eGFP-CaM-uTEV1Δ

Use

AAV

Tags

eGFP, V5

Expression

Mammalian

Mutation

S219V mutation improves stability and S153N impro…

Promoter

Synapsin

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano30/pcDNA3

Plasmid

#51963

Purpose

mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano30

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PR promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEM-GECO1

Plasmid

#32463

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 8.

Insert

GEM-GECO1

Use

Lab constructed

Tags

6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pTorPE-FRISZ

Plasmid

#176888

Purpose

Far-red fluorescent zinc indicator FRISZ in pTorPE vector

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

FRISZ

Use

Tags

Periplasmic expression TorA tag and Twin-Strep-ta…

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M7NS_AmpR

Plasmid

#119157

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-2T SHP1 CS

Plasmid

#8595

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C453S (catalytically inactive)

Promoter

Available since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Article

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Insert

BRAC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Article

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Use

Tags

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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NK-1 GFP

Plasmid

#24508

Depositor

Ron Vale

Article

Case et al Cell. 1997 Sep 5. 90(5):959-66.

Insert

Drosophila NCD, human kinesin

Use

Tags

GFP and His

Expression

Bacterial

Mutation

NK-1 is a chimera where the entire superfamily-co…

Promoter

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup35-EcoRI

Plasmid

#87332

Purpose

To express shRNA against human Nup35

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup35

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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TTVTFLPTPSLILQT_epitope

Plasmid

#165021

Purpose

Expression of CMV UL44 (38-52) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (38-52)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEX-GECO1

Plasmid

#32464

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 8.

Insert

GEX-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-nirButterfly

Plasmid

#136590

Purpose

Expression of a genetically-encoded voltage indicator nirButterfly

Depositor

Vladislav Verkhusha

Article

Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

Insert

nirButterfly

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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nPEX-LV

Plasmid

#12119

Depositor

Inder Verma

Article

Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

Insert

Matrix Metalloproteinase 2 (Mmp2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

noncatalytic fragment of MMP2 amplified via PCR, …

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
AAV-Y-GECO1

Plasmid

#55767

Purpose

Express Y-GECO1 in neurons

Depositor

Robert Campbell

Article

Zhao et al Integr Biol (Camb). 2014 May 19.

Insert

Y-GECO1.0

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 LacZ

Plasmid

#123292

Purpose

Mammalian expression of control truncated LacZ

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

LacZ

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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