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Plasmid#213205PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCRL2 (CCRL2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR3-DuET
Plasmid#213199PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR3 (CCR3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
C5A-DuET
Plasmid#213192PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertC5A (C5AR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCKAR-DuET
Plasmid#213195PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCKAR (CCKAR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCKBR-DuET
Plasmid#213196PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCKBR (CCKBR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AVPR1B-DuET
Plasmid#213187PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertAVPR1B (AVPR1B Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AVPR2-DuET
Plasmid#213188PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertAVPR2 (AVPR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADRB1-DuET
Plasmid#213180PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertADRB1 (ADRB1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADRA2B-DuET
Plasmid#213178PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertADRA2B (ADRA2B Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADRA2C-DuET
Plasmid#213179PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertADRA2C (ADRA2C Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
VIPR2-DuET
Plasmid#213387PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertVIPR2 (VIPR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 3C-CVB3
Plasmid#202077PurposeBacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 3C protease with HisTag
UseTags6xHis-tagExpressionBacterial, Insect, and Mamm…MutationPromoterpolh, CAG, T7Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBVboostFGII WPRE 2A-CVB3
Plasmid#202076PurposeBacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.DepositorInsertCVB3 2A protease with HisTag
UseTags6xHis-tagExpressionBacterial, Insect, and Mamm…MutationPromoterpolh, CAG, T7Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL5 sgRNA(MS2)-MS2-hA3a D131A
Plasmid#112131Purposeempty sgRNA cloning vector with MS2-humanA3a D131ADepositorInserthuman Apobec3a D131A mutant (APOBEC3A Human)
UseCRISPRTagsExpressionMammalianMutationD131A mutant in hA3aPromoterhU6,CBhAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE
Plasmid#205283PurposeGCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020)DepositorInsertGCaMP8f
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1_heavy_CoVMab7
Plasmid#194605PurposeMammalian expression of CoVMab7 heavy chainDepositorInsertCoVMab7 heavy chain (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1_heavy_CoVMab4
Plasmid#194603PurposeMammalian expression of CoVMab4 heavy chainDepositorInsertCoVMab4 heavy chain (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1_kappa_CoVMab7
Plasmid#194606PurposeMammalian expression of CoVMab7 light chainDepositorInsertCoVMab7 light chain (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1_kappa_CoVMab4
Plasmid#194604PurposeMammalian expression of CoVMab4 light chainDepositorInsertCoVMab4 light chain (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only