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Plasmid#167577PurposeExpress the ATP sensor GRAB_ATP1.0 in neuronsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
Plasmid#129631PurposeVisualization of Akt and ERK activityDepositorInsertH2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(mito).cpSFGFP.HaloTag
Plasmid#214925PurposeExpresses mitochondrially targeted non-responsive controlDepositorInsert(mito).cpSFGFP.HaloTag
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-X1-Neo-GFP-FAM134B
Plasmid#109026Purposestable lentiviral expression of cDNADepositorInsertFAM134B (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-hHDAC3
Plasmid#98591Purpose3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.DepositorInsertdCAS9-HDAC3 (HDAC3 Synthetic, S. pyogenes, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0238
Plasmid#142490PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-mStayGold (E138D)
Plasmid#211361PurposeBacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
UseTags10xHis-tagExpressionBacterialMutationE138DPromoterT7Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1-GFP
Plasmid#51407PurposeIs a biosensor for PI(4,5)P2DepositorInsertPH-PLCD1 (aa 1–170) (PLCD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB-gDA3h
Plasmid#208699PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h in neuronsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3h
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21a-alpha-synuclein
Plasmid#51486PurposeInducible expression of human alpha-synuclein in E.coliDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
hBax C3-EGFP
Plasmid#19741DepositorInsertBax (BAX Human)
UseTagsGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
GST-RHâ–²-MNase
Plasmid#136292PurposeExpression of catalytic inactive RNase H fused to micrococcal nucleaseDepositorInsertRNase HI-D10R-E48R and MNase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_FLAG-Mma PylRS
Plasmid#140009PurposePlasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8m
Plasmid#162372PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8m
UseTags6xHisExpressionMammalianMutationA25G F286YPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-hygro
Plasmid#160090PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
DT960
Plasmid#80412PurposeExpresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_Keima
Plasmid#127140DepositorInsertRPS3_Keima (RPS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only