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2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

Plasmid

#172224

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRS315-PGK-SEIPIN1

Plasmid

#96986

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

Phosphoglycerate Kinase (PGK) promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 CrkII

Plasmid

#50731

Purpose

mammalian expression of CrkII

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII (CRK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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Flag Snail 4SA

Plasmid

#16220

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

4SA: S108A, S112A, S116A, S120A

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pGSTag BimL

Plasmid

#24251

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (BCL2L11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pTH760-CEN-mCherry_v3

Plasmid

#40598

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences

Use

Tags

Expression

Yeast

Mutation

mCherry ORF partially codon optimised for yeast e…

Promoter

GPD (TDH3)

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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HIPK1-myc-his

Plasmid

#1850

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

HIPK1 (Hipk1 Mouse)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pCEP4_OCT4_T234E_S235E

Plasmid

#40631

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

T234E_S235E

Promoter

EF1-alpha

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pCEP4_OCT4_T234A_S235A

Plasmid

#40630

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1_S234A_T235A (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

S234A_T235A

Promoter

EF1-alpha

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pYZ_3WJrep_N10_trigger

Plasmid

#132728

Purpose

T7 RNAP-driven expression of 3WJ repressor index 10 trigger RNA

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N10_trigger

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_3WJrep_N10_switch

Plasmid

#132724

Purpose

T7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporter

Depositor

Peng Yin, Alexander Green

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_N10_switch

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

stop at beginning of last exon

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEF6 rglut1 HA complete

Plasmid

#89572

Purpose

rglut1 HA complete

Depositor

Jeffrey Rathmell

Article

Wieman et al Mol Biol Cell. 2007 Apr;18(4):1437-46. E

Insert

pEF6 rglut1 HA complete (Slc2a1 Rat)

Use

Tags

V5-His A, B, C and rglut1 HA complete

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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3486 pBABE Bid p22 GFP

Plasmid

#8777

Depositor

Stanley Korsmeyer

Article

Zha et al Science 2000 Dec 1;290(5497):1761-5.

Insert

Bid p22 (Bid Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
aq-CFP

Plasmid

#36073

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein subunit alpha q (Gnaq Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between amino acid 124 and 125

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-MKRN1 WT

Plasmid

#78766

Purpose

To detect localization of MKRN1 in live mammalian cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

MKRN1 (MKRN1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
ManII-SBP-mCherry_puromycin

Plasmid

#65261

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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ATF4 18: RLuc.5’ATF4.GFP

Plasmid

#21864

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
DAXX-myc

Plasmid

#1851

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only