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Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet2 CD
Plasmid#72219PurposeExpress flag-tagged mouse Tet2 catalytic domain.DepositorInsertTen-Eleven Translocation 2 (Tet2 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET2 catalytic domain.PromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
Plasmid#135454PurposeBacterial expression of TEVΔ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability. Truncated form…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
V51 pIRESpuro-GLUE (pGLUE)
Plasmid#15100Purposetandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tagsDepositorTypeEmpty backboneUseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pcDNA3
Plasmid#51961Purposemammalian expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nMDepositorInsertYellow Cameleon-Nano15
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Clomeleon
Plasmid#203723PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentrationDepositorInsertcyan fluorescent protein linked yellow florescent protein
UseTagsExpressionBacterial and MammalianMutationCyan Fluorescent Protein, Topaz Fluorescent Prote…PromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano65/pcDNA3
Plasmid#51965Purposemammalian expression of Yellow Cameleon-Nano65, an ultrasensitive Ca2+ indicator with Kd = 65 nMDepositorInsertYellow Cameleon-Nano65
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRU5HT1-inLuc
Plasmid#58954PurposeHTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.DepositorInsertinLuc
UseRetroviralTagsExpressionMutationPromoterCMV/R/U5Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-1P
Plasmid#202607PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-1P
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA
Plasmid#194247PurposeExpresses 3 miRNAs targeting human alpha-SynucleinDepositorInsertmiRaSynuclein(human) (SNCA Human)
UseAAV and RNAiTagsExpressionMutationPromoterCAGAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-FRISZ
Plasmid#176887PurposeFar-red fluorescent zinc indicator FRISZ in pDisplayDepositorInsertFRISZ
UseTagspDisplay backbone elements for export and membran…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_M4NS_AmpR
Plasmid#119156PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed regionDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
UseCrisprTagsExpressionMutationPromoterJ23119Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
LOTUS-V/pcDNA3
Plasmid#87127PurposeBioluminescent and ratiometric voltage indicatorDepositorInsertLOTUS-V
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHUC-JEDI-1P
Plasmid#202619PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P under the HuC promoter for pan neuronal expression in zebrafish; JEDI-1P sequence codon-optimized for zebrafish expressionDepositorInsertJEDI-1P
UseZebrafish expressionTagsExpressionMutationPromoterHuCAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_1.2μ-pcDNA3
Plasmid#111926PurposeCyan color bright luminescent calcium indicator with intermediate affinityDepositorInsertCeNL(Ca2+)_1.2μ
UseTagsExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
OeNL(Ca2+)_18μ-pcDNA3
Plasmid#111928PurposeOrange color bright luminescent calcium indicator with intermediate affinityDepositorInsertOeNL(Ca2+)_18μ
UseTagsExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pprm1-luciferase; his-3
Plasmid#137728PurposeCommunication-activated luciferase in Neurospora crassaDepositorInsertsLuciferase
Pprm-1 Promoter
UseLuciferase; Neurospora crassa expressionTagsExpressionMutationCodon-optimized for N. crassaPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_19μ-pcDNA3
Plasmid#111927PurposeCyan color bright luminescent calcium indicator with intermediate affinityDepositorInsertCeNL(Ca2+)_19μ
UseTagsExpressionMammalianMutationE67D, F92W, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only