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pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
UseTagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
hROSA26 CRISPR-pX330
Plasmid#105927PurposehROSA26 targeting CRISPR plasmidDepositorInserthROSA26 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK231 (AAVS1 CMV-MCS-PURO)
Plasmid#105924PurposeDonor for integration at the human AAVS1 locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIL-eGFP
Plasmid#52675PurposeshRNA silencing plasmid with eGFP transfection markerDepositorTypeEmpty backboneUseRNAiTagsCMV-EGFPExpressionMutationPromoterCMV and U6Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Nup88
Plasmid#64283PurposeGFP-tagged mammalian expression of full-length Nup88DepositorInsertNup88 (NUP88 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Paxillin-pEGFP
Plasmid#15233DepositorInsertpaxillin (PXN Chicken)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJJB302
Plasmid#107694PurposeOsActin Promoter-LwaCas13a-HSP TerminatorDepositorInsertCas13a
UseTagsExpressionPlantMutationPromoterOsActinAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAureus-TnCAM
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGCG26
Plasmid#82371PurposeYFP expressed from pJ23119 in the glpK locus with gentamicin resistanceDepositorInsertYFP
UseTagsExpressionMutationPromoterPJ23119Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas49
Plasmid#82391PurposerbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpKDepositorInsertrbcL-GFP
UseTagsExpressionMutationPromoterPccmK2Available sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T2-Rheb
Plasmid#15889DepositorInsertRheb (RHEB Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSETT4gap
Plasmid#153413PurposeφC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassetteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotergapDH promoter from Eggerthella lentaAvailable sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
RSV-betaGal
Plasmid#24058DepositorInsertLacZ (lacZ E. coli)
UseTagsRSV promoterExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nup88
Plasmid#64281PurposeMammalian expression of full-length Nup88DepositorInsertNup88 (NUP88 Human)
UseTagshis and mycExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDYU1G/γ-tubulin-mEGFP
Plasmid#154301PurposeExpresses γ-tubulin–mEGFP from actin15 promoter in Dictyostelium discoideumDepositorInsertγ-tubulin
UseDictyostelium expressionTagsmEGFPExpressionMutationPromoteractin15Available sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mWnt2
Plasmid#42274DepositorInsertWnt2 (Wnt2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbPpep-CHis
Plasmid#48243PurposeFirst 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domainDepositorInsertClbP-pep
UseTags6-HisExpressionBacterialMutationFirst 375 amino acids of the peptidase, which con…PromoterT7Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only
