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Showing: 3961 - 4000 of 7564 results

  11. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-Kras
    Use
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  16. pClneoFH-let60 G12V

    Plasmid
    #66860
    Purpose
    Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells
    Depositor
    Yutaka Hata
    Article
    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do
    Insert
    Let-60 (let-60 Nematode)
    Use
    Tags
    FLAG-His6
    Expression
    Mammalian
    Mutation
    Glycine 12 is mutated to valine
    Promoter
    CMV
    Available since
    Aug. 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pOCC177

    Plasmid
    #118886
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    Use
    Tags
    HIS6-MBP, cleavable with 3C protease and SNAP tag…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118886-plasmid-map-sequence-id-232379

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  23. pOCC210

    Plasmid
    #118896
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and SNAP tag, cle…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118896-plasmid-map-sequence-id-232311

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  24. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Use
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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  27. pcDNA3-mCherry-LKB1(S431A)

    Plasmid
    #84641
    Purpose
    mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    mCherry-LBK1(S431A) (STK11 Human, Synthetic)
    Use
    Tags
    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Mutation
    PKA phosphorylation site Serine 428 mutated to Al…
    Promoter
    CMV
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    84641-plasmid-map-sequence-id-176205

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  28. pTT610

    Plasmid
    #101742
    Purpose
    encoding SecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion protein and SecE and SecG from Thermus thermophilus HB8
    Depositor
    Tomoya Tsukazaki
    Article
    Sugano et al PLoS One. 2017 Aug 18;12(8):e0183434. do
    Insert
    Thermus thermophilus HB8 SecY-A/SecE/SecG (secE, secY Thermus thermophilus HB8)
    Use
    Tags
    His10
    Expression
    Bacterial
    Mutation
    SecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion p…
    Promoter
    Lac
    Available since
    Nov. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    101742-plasmid-map-sequence-id-197570

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  29. p5A7-T

    Plasmid
    #92020
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92020-plasmid-map-sequence-id-190036

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  30. pMpGWB419

    Plasmid
    #68684
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68684-plasmid-map-sequence-id-121817

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  31. pMpGWB422

    Plasmid
    #68687
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68687-plasmid-map-sequence-id-121835

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  32. pMpGWB322

    Plasmid
    #68650
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68650-plasmid-map-sequence-id-131805

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Showing: 3961 - 4000 of 7564 results