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pT7-EGFP-C1-HsRCK-RFQNRtoEDAAA-shRNAres_AC

Plasmid

#148454

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA-shRNAres (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

Plasmid

#148420

Purpose

Mammalian Expression of HsRck_296-472-10xmut

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-10xmut (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK_E

Plasmid

#146214

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-K298M-Y419F-Y530F

Plasmid

#140321

Purpose

Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

K298M-Y419F-Y530F

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-K298M-Y419F-Y530F

Plasmid

#140320

Purpose

Expresses HA-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

K298M-Y419F-Y530F

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-cSrc-SH2-AviTag

Plasmid

#214230

Purpose

Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

Insert

cSrc kinase SH2 domain (SRC Human)

Use

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-E236Q_E

Plasmid

#146212

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-E236Q (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-228-GST-HsRCK-K135A_E

Plasmid

#146213

Purpose

Bacterial Expression of HsEDC3_192-228-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-228-HsRck-K135A (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-E236Q_E

Plasmid

#146215

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-E236Q

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-E236Q (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508-GST-HsRCK-K135A_E

Plasmid

#146216

Purpose

Bacterial Expression of HsEDC3_192-508-HsRck-K135A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsEDC3_192-508-HsRck-K135A (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsRCK_296-472-HsPatL1_1-111 (MCSII)_D

Plasmid

#146105

Purpose

Bacterial Expression of HsRck_296-472-HsPatL1_1-111

Depositor

Elisa Izaurralde

Insert

HsRck_296-472-HsPatL1_1-111 (DDX6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsRCK_296-472-HsPatL1_1-173 (MCSII)_D

Plasmid

#146106

Purpose

Bacterial Expression of HsRck_296-472-HsPatL1_1-173

Depositor

Elisa Izaurralde

Insert

HsRck_296-472-HsPatL1_1-173 (DDX6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsRCK_296-472-HsPatL1_1-398 (MCSII)_D

Plasmid

#146107

Purpose

Bacterial Expression of HsRck_296-472-HsPatL1_1-398

Depositor

Elisa Izaurralde

Insert

HsRck_296-472-HsPatL1_1-398 (DDX6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508del231-263_GST-HsRCK_70-472_D

Plasmid

#146110

Purpose

Bacterial Expression of HsEDC3_192-508del231-263-HsRck_70-472

Depositor

Elisa Izaurralde

Insert

HsEDC3_192-508del231-263-HsRck_70-472 (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508del231-263_GST-HsRCK_296-472_D

Plasmid

#146111

Purpose

Bacterial Expression of HsEDC3_192-508del231-263-HsRck_296-472

Depositor

Elisa Izaurralde

Insert

HsEDC3_192-508del231-263-HsRck_296-472 (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3 _192-508del231-254_GST-HsRCK_D

Plasmid

#146112

Purpose

Bacterial Expression of HsEDC3_192-508del231-254-HsRck

Depositor

Elisa Izaurralde

Insert

HsEDC3_192-508del231-254-HsRck (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsEDC3_192-508del231-254_GST-HsRCK_70-472_D

Plasmid

#146108

Purpose

Bacterial Expression of HsEDC3_192-508del231-254-HsRck_70-472

Depositor

Elisa Izaurralde

Insert

HsEDC3_192-508del231-254-HsRck_70-472 (EDC3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

Plasmid

#210508

Purpose

expresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cells

Depositor

Dongmin Lee

Insert

NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

Use

Tags

GB1

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pM5MF529

Plasmid

#17686

Depositor

David Shalloway

Article

Lin et al Oncogene. 1995 Jan 19. 10(2):401-5.

Insert

c-src Y529F (Src Mouse)

Use

Tags

Expression

Mammalian

Mutation

Tyr 529 changed to Phe .

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pM5HHB5

Plasmid

#17674

Depositor

David Shalloway

Article

Kmiecik et al Cell. 1987 Apr 10. 49(1):65-73.

Insert

c-src (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only