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We narrowed to 526 results for: prime editing

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pCMV_T7-PE2-Nuclease

Plasmid

#171997

Purpose

Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CMV_T7-Cas9-RT

Use

Tags

Expression

Mammalian

Mutation

GC to CA point mutation in SpCas9 to restore nucl…

Promoter

CMV for Cas9, U6 for gRNAs

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_KCNA1-EGFP

Plasmid

#159787

Purpose

S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting KCNA1 gene (KCNA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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TS-PE2-C-WPRE

Plasmid

#176650

Purpose

Expresses C-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

C-terminus of PE2
Artificial SA
WPRE
bGH poly(A)

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

None

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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TS-PE2-N

Plasmid

#176649

Purpose

Expresses N-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

N-terminus of PE2
Artificial SD

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV and None

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_FANCF-EGFP

Plasmid

#159785

Purpose

S. pyogenes pegRNA for C to A mutation at the FANCF site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting FANCF gene (FANCF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_HOXD13_CtoT-EGFP

Plasmid

#159792

Purpose

S. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_HOXD13_CtoG-EGFP

Plasmid

#159791

Purpose

S. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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hCtRNA_VT

Plasmid

#186716

Purpose

Vector template for DAP array

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

hCtRNA and gRNA scaffold

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hCtRNA

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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hCtRNA_FT

Plasmid

#186715

Purpose

Fragment template for DAP array

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

gRNA scaffold and hCtRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hCtRNA

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pICH47742::p35S-ePEmax3::tEURb7

Plasmid

#227692

Purpose

Golden Gate level 1, position 2, ePEmax3 expression cassette.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

ePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pegRNA_GBA-4

Plasmid

#199274

Purpose

pegRNA used to correct GBA (c. 1226 A > G) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

GBA 1226GtoA pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA-GBA-10

Plasmid

#199271

Purpose

pegRNA used to induce GBA (c. 1226 A > G) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

GBA 1226AtoG pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA_LMNA_CR-2-5

Plasmid

#199275

Purpose

pegRNA used to correct LMNA (c.1824C > T) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA 1824TtoC pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA_LMNA-1

Plasmid

#199273

Purpose

pegRNA used to induce LMNA (c.1824C > T) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LMNA 1824CtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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Lsg-LRRK2-1

Plasmid

#199277

Purpose

nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 nick

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA_LRRK2-3

Plasmid

#199272

Purpose

pegRNA used to induce LRRK2 (c. 6055 G > A) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 6055GtoA pegRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-hMLH1dn

Plasmid

#222997

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-hMLH1dn

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Deletion of residues 754-756 in MLH1 as described…

Promoter

CMV

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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nLbCas12a

Plasmid

#213745

Purpose

For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

nLbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, R1138A

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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LbCas12a-crRNA

Plasmid

#213753

Purpose

For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a-crRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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LbCas12a-D156R

Plasmid

#213744

Purpose

For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

LbCas12a

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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