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Plasmid#187574PurposeExpress FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cellsDepositorInsertRNPS1 (RNPS1 Human)
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term
Plasmid#182925PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneUseTagsAPEX2 and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1
Plasmid#187579PurposeExpress FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cellsDepositorInsertPSPC1 (PSPC1 Human)
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-APEX2-Ires-eGFP-PAC
Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term
Plasmid#182926PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneUseTagsAPEX2 and FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBL
Plasmid#187577PurposeExpress FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cellsDepositorInsertFBL (FBL Human)
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-APEX2-Venus
Plasmid#73467PurposeExpresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.DepositorInsertAPEX2-Venus
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1
Plasmid#187586PurposeExpress FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cellsDepositorInsertEWSR1 (EWSR1 Human)
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 FLAG-APEX2-P4C-IRES-mNeonGreen
Plasmid#218645PurposeGateway shuttling vector for APEX2-tagged P4C, a P4C biosensorDepositorInsertSidC(P4C)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC APEX2-3xFLAG-IRES-eGFP
Plasmid#214983PurposeCytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).DepositorInsertAPEX2 (APX2 Glycine max (soybean))
UseLentiviralTags3xFLAGExpressionMutationPromoterUBCAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Cox4-GFP-APEX2-Ura
Plasmid#207062PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpCOX4Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Snf7-4V5-APEX2-URA
Plasmid#207065PurposeModerate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.DepositorInsertSnf7
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pERG6-Pex3-4V5-APEX2-URA
Plasmid#207068PurposeModerate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.DepositorInsertPex3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpERG6Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pLSP1-Pex3-4V5-APEX2-URA
Plasmid#207069PurposeModerate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.DepositorInsertPex3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpLSP1Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Tgl3-4V5-APEX2-URA
Plasmid#207059PurposeExpressing Tgl3-4V5-APEX2. Insufficient signal for EM.DepositorInsertTgl3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpTGL3Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Pex3-4V5-APEX2-URA
Plasmid#207057PurposeExpressing Pex3-4V5-APEX2. Insufficient signal for EM.DepositorInsertPEX3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpPEX3Available sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Emc1-GFP-APEX2-Ura
Plasmid#207074PurposeExpressing Emc1-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertEmc1
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Cox4-GFP-APEX2-Ura
Plasmid#207075PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53
Plasmid#187578PurposeExpress FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cellsDepositorInsertWRAP53 (WRAP53 Human)
UseTagsAPEX2-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-P2A-APEX2-VHH[GFP]
Plasmid#126749PurposeExpression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJDepositorInsertVHH[EGFP]
UseZebrafish muscle expression cloneTagsmKate2-P2A-APEX2ExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only