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pCRISPRainbow-DONOR1

Plasmid

#75398

Purpose

CRISPRainbow-DONOR1

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Cm(R)-CcdB

Use

CRISPR

Tags

no

Expression

Mutation

Promoter

Bacteria

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCR3.1-Luc

Plasmid

#106457

Purpose

luciferase reporter, CMV promoter

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

luciferase reporter, CMV promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

WT promoter

Promoter

Available since

Nov. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-NanoLuc

Plasmid

#67178

Purpose

CRISPaint universal donor for NanoLuc fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

NanoLuc

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk12_Untagged

Plasmid

#127176

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

None

Expression

Mutation

Promoter

None

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-18a

Plasmid

#61326

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCR-HA-IKKbeta

Plasmid

#15470

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
pCRISPaint-TagGFP2

Plasmid

#67166

Purpose

CRISPaint universal donor for TagGFP2 fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagGFP2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-SBP

Plasmid

#67173

Purpose

CRISPaint universal donor for streptavidin binding peptide fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

SBP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII p15

Plasmid

#16454

Depositor

Bert Vogelstein

Article

Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

Insert

p15 (CDKN2B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pAC1817_pCR8-gSMN2-EX

Plasmid

#118651

Purpose

Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-EX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8-PHF19

Plasmid

#68864

Purpose

GW entry vector for PHF19

Depositor

Adrian Bracken

Article

Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

Insert

PHF19 (PHF19 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCRISPReporter-mCherry

Plasmid

#65008

Purpose

E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.

Depositor

Mattheos Koffas

Article

Cress et al ACS Synth Biol. 2015 Mar 30.

Insert

mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

User-defined

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCRU5-inGFPt

Plasmid

#60236

Purpose

HTLV-1 based transfer vector encoding gfp-turbo reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inGFPt

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pCR3.1-cGK1

Plasmid

#106452

Purpose

expression of murine cyclic GMP-dependent kinase

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

cyclic GMP-dependent kinase (Prkg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xFlag

Plasmid

#67170

Purpose

CRISPaint universal donor for flag fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

3xFlag

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRU5HT1-inLuc

Plasmid

#58954

Purpose

HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

Depositor

Dmitriy Mazurov, David Derse

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Retroviral

Tags

Expression

Mutation

Promoter

CMV/R/U5

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc

Plasmid

#107982

Purpose

expression of OPN splice variant c

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

osteopontin splice variant c (SPP1 Human)

Use

Tags

Expression

Mammalian

Mutation

splice variant

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCR2.1-myco

Plasmid

#36870

Depositor

Mario Chin

Insert

myco

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-Sth1Cas9

Plasmid

#129552

Purpose

Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

Depositor

Mingzi M. Zhang, Fong Tian Wong

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

Sth1Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

rpsL(XC)-BbsI

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8 PALI1

Plasmid

#114442

Purpose

Entry vector for gateway cloning containing human PALI1 coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

PALI1 (LCOR Human)

Use

Gateway entry cloning

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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