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Plasmid#67105PurposeStandard for cell-free expression benchmarking.DepositorInsertBMPR1A (BMPR1A Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 EHD2
Plasmid#67107PurposeStandard for cell-free expression benchmarking.DepositorInsertEHD2 (EHD2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationS320T, E384D, T387A, H388Q, T405V, V407A, H418R, …PromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P REV3L_1
Plasmid#160797PurposeSuppress REV3LDepositorInsertshREV3L_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIS-MDH1-HIS
Plasmid#184558PurposeBacterial expression of human MDH1 with HIS TagDepositorInsertMalate dehydrogenase 1 (MDH1 Human)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2333_Tier3(Lenti_inverse)-YPet_Puro
Plasmid#169664PurposeTier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)DepositorInsertPRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / RPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHRASLS.2
Plasmid#110324PurposeTRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2DepositorInsertHRASLS (PLAAT1 Human, Homo sapiens)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shHRASLS.1
Plasmid#110323PurposeTRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2DepositorInsertHRASLS (PLAAT1 Human, Homo sapiens)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPTG-GG-sg2+sg3
Plasmid#127199PurposePlasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoterDepositorInserttRNA-gRNA
UseEmpty sgrna plasmidTagsExpressionMammalianMutationPromoterU6Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA
Plasmid#184549PurposeRetroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tagDepositorUseRetroviralTags3xFLAG and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUltra-MDH1-ME1
Plasmid#184465PurposeLentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)DepositorUseLentiviralTagsfused to T2AExpressionMammalianMutationdeletion of stop codonPromoterUbCAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM299
Plasmid#224258PurposeMCMUbHisDepositorInsertMC-Ub-3C-His
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM301
Plasmid#226134PurposeExpression of MC-Ub-SH3-3C-His6DepositorInsertMC-Ub-SH3-3C-His6
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM298
Plasmid#224257PurposeMCM WT ubiquitinDepositorInsertMCM Ubiquitin
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM305
Plasmid#226137PurposeExpression of His6-mEos3.2-UbK48RDepositorInsertHis6-mEos3.2-UbK48R
UseTagsExpressionBacterialMutationK48RPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM302
Plasmid#226135PurposeExpression of MC-Ub-UbK48R-SH3-3C-His6DepositorInsertMC-Ub-UbK48R-SH3-3C-His6
UseTagsExpressionBacterialMutationK48RPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM303
Plasmid#226136PurposeExpression of His6-GG-UbK48RDepositorInsertHis6-GG-UbK48R
UseTagsExpressionBacterialMutationK48RPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM294
Plasmid#226130PurposeExpression of Ufd1-3C-His6DepositorInsertUfd1-3C-His6
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T1 mouse PARG (439-959)
Plasmid#132615Purposeexpresses mouse PARG(439-959) in E. coli.DepositorInsertPARG (439-959)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM292
Plasmid#226129PurposeExpression of His6-SUMO-Ub-mEos3.2-intein-CBDDepositorInsertHis6-SUMO-Ub-mEos3.2-intein-CBD
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only