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M57 pPET28a-TEV-full-length human beta catenin

Plasmid

#17198

Depositor

Randall Moon

Insert

beta catenin (CTNNB1 Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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M58 pPET28b-TEV-full-length zebrafish beta catenin

Plasmid

#17199

Depositor

Randall Moon

Insert

beta catenin (ctnnb1 Zebrafish)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
MSCV-(mcs) human CSF1 full length-IRES-Puro

Plasmid

#86797

Purpose

retroviral expression of CSF-1

Depositor

Charles Sherr, Martine Roussel

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-Puro

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

Plasmid

#217013

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7(W132R, S164D, T170E) (CDK7 Human)

Use

Tags

TEV cleavable his tag

Expression

Insect

Mutation

CDK7(W132R, S164D, T170E)

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

Plasmid

#199566

Purpose

Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

Depositor

Andrea Cimarelli, Yuxin Song

Article

Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)

Use

Retroviral; Allows for puromycin selection

Tags

Flag

Expression

Mutation

codon optimized

Promoter

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

Plasmid

#217012

Purpose

Expression of CDK7 in insect cells

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK7 (S164D, T170E)_del327-346 (CDK7 Human)

Use

Tags

TEV cleavable his tag

Expression

Insect

Mutation

(S164D, T170E)_del327-346

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-(pbabe puro mcs)-human CSF-1 full length (CSF4)-IRES-GFP

Plasmid

#86796

Purpose

retroviral expression of human full length CSF-1(256)

Depositor

Charles Sherr, Martine Roussel

Article

Heard et al Oncogene Res. 1987 Sep-Oct;1(4):423-40.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCORE-UK

Plasmid

#72238

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
kanMX4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pZS2-123

Plasmid

#26598

Depositor

Michael Elowitz

Article

Cox et al J Biol Eng. 2010 . 4():10.

Insert

Synthetic

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCORE-UH

Plasmid

#72239

Purpose

Contains the CORE cassette with KlURA3 and hyg markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
hyg

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-melR-deGFP-T500

Plasmid

#45396

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

melibiose repressor

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pBADmod1-linker-deGFP

Plasmid

#45397

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pB33eCPX

Plasmid

#23336

Depositor

Patrick Daugherty

Article

Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

Insert

Enhanced Circularly Permuted Outer Membrane Protein X

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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pChlr-pNEB193

Plasmid

#52136

Purpose

A backbone plasmid for creation of additional PUF repeat modules

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
SW5

Plasmid

#40108

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Bistable switch using dynamic degradation

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pSLQ8001 - pCMV-R8.91 (D64V)

Plasmid

#202687

Purpose

R8.91 plasmid with mutated integrase (D64V)

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

gag pol

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

D64V

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pIYC04

Plasmid

#64741

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ8079 - IL2RG GFP-P2A CLIP Donor

Plasmid

#202690

Purpose

CLIP donor template for integrating GFP into IL2RG locus.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Use

Tags

Expression

Mutation

Promoter

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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PRM-GFP

Plasmid

#40127

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

CI inducible sfGFP

Use

Synthetic Biology

Tags

LVA ssrA tag

Expression

Bacterial

Mutation

Promoter

pRM

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pMU102

Plasmid

#61805

Purpose

Cloning and expression vector for Clostridium thermocellum

Depositor

Lee Lynd

Article

Olson et al J Biol Eng. 2012 May 11;6(1):5. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge