Showing: 181 - 200 of 204 results
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Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
UseTagsNone.ExpressionBacterial and MammalianMutationPromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE191.pa
Plasmid#134931PurposeGEF-H1 biosensor that has GEF activity.DepositorInsertGEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
UseTagsNone.ExpressionBacterial and MammalianMutationPromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-6080NES(Epac-sensor)
Plasmid#162487PurposehyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertEpac-sensor (RAPGEF3 Human, Synthetic)
UseTagsYPet and Turquoise2GLExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RasAR-NES
Plasmid#205567PurposeGenetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.DepositorInsertRasAR-NES (RAF1 Human, Synthetic)
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RasAR(R89L)-NES
Plasmid#205568PurposeRasAR negative-control mutant plasmid. Targeted to cytosol.DepositorInsertRasAR(R89L)-NES (RAF1 Human, Synthetic)
UseTagsNuclear export signal (NES)ExpressionMammalianMutationArg 89 mutated to Leu in Raf1 RBD region.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET15b-M1C
Plasmid#107085PurposeM. sedula PCNA1 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA1 fused to CyPet
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET15b-M2C
Plasmid#107086PurposeM. sedula PCNA2 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA2 fused to CyPet
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET15b-M3C
Plasmid#107087PurposeM. sedula PCNA3 fused at the N-terminus of CyPetDepositorInsertM. sedula PCNA3 fused to CyPet
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET15b-S1C
Plasmid#107088PurposeS. solfataricus PCNA1 fused at the N-terminus of CyPetDepositorInsertS. solfataricus PCNA1 fused to CyPet
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pET15b-S3C
Plasmid#107090PurposeS. solfataricus PCNA3 fused at the N-terminus of CyPetDepositorInsertS. solfataricus PCNA3 fused to CyPet
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
JH05
Plasmid#73516PurposemYPet_PH pCFJ150 - contain C. elegans codon optimized mYPetDepositorInsertpmex-5::mPYet::PH::tbb-2
UseTagsmYPetExpressionMutationPromotermex-5AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Golgi-RasAR
Plasmid#205570PurposeGolgi membrane-targeted RasAR.DepositorUseTagsGolgin B1 (Giantin) residues 3131-3259ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
UseTagsExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-CFP
Plasmid#90103PurposeCFP control expression plasmidDepositorInsertCFP
UseBroad-host rangeTagsExpressionBacterialMutationPromoterPtacAvailabilityAcademic Institutions and Nonprofits only -
LT2_15_UPRpro_X2site
Plasmid#194427PurposePlasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein ResponseDepositorInsertUPRpro
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationNAPromoterAvailabilityAcademic Institutions and Nonprofits only -
LT2_33_PyruPro_X2site
Plasmid#194429PurposePlasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)DepositorInsertPyruPro
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationNAPromoterAvailabilityAcademic Institutions and Nonprofits only -
LT2_7_OxPro
Plasmid#177709PurposePlasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)DepositorInsertspTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 181 - 200 of 204 results