Addgene

Showing: 181 - 200 of 204 results

Results per page:

GEF-H1 FLARE212.pa

Plasmid

#134930

Purpose

GEF-H1 biosensor that does not have GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)

Use

Tags

None.

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and T7

Availability

Academic Institutions and Nonprofits only

Enlarge
GEF-H1 FLARE191.pa

Plasmid

#134931

Purpose

GEF-H1 biosensor that has GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)

Use

Tags

None.

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBbsr-6080NES(Epac-sensor)

Plasmid

#162487

Purpose

hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Epac-sensor (RAPGEF3 Human, Synthetic)

Use

Tags

YPet and Turquoise2GL

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-RasAR-NES

Plasmid

#205567

Purpose

Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR-NES (RAF1 Human, Synthetic)

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-RasAR(R89L)-NES

Plasmid

#205568

Purpose

RasAR negative-control mutant plasmid. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR(R89L)-NES (RAF1 Human, Synthetic)

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Arg 89 mutated to Leu in Raf1 RBD region.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-M1C

Plasmid

#107085

Purpose

M. sedula PCNA1 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA1 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-M2C

Plasmid

#107086

Purpose

M. sedula PCNA2 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA2 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-M3C

Plasmid

#107087

Purpose

M. sedula PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-S1C

Plasmid

#107088

Purpose

S. solfataricus PCNA1 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-S3C

Plasmid

#107090

Purpose

S. solfataricus PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
JH05

Plasmid

#73516

Purpose

mYPet_PH pCFJ150 - contain C. elegans codon optimized mYPet

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mPYet::PH::tbb-2

Use

Tags

mYPet

Expression

Mutation

Promoter

mex-5

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-Golgi-RasAR

Plasmid

#205570

Purpose

Golgi membrane-targeted RasAR.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

Golgi-RasAR (RAF1, GOLGB1 Human, Synthetic)

Use

Tags

Golgin B1 (Giantin) residues 3131-3259

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
H3K9me3 biosensor

Plasmid

#120802

Purpose

FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
K9L mutant of H3K9me3 biosensor

Plasmid

#120807

Purpose

FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)

Use

Tags

Expression

Mammalian

Mutation

lysine 9 is mutated to leucine 9 on histone H3

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
W45A mutant of H3K9me3 biosensor

Plasmid

#120808

Purpose

FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3

Use

Tags

Expression

Mammalian

Mutation

Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
AIMTOR T757

Plasmid

#140828

Purpose

AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

Depositor

Anne Bonnieu

Article

Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

Insert

AIMTOR T757 (ULK1 Human)

Use

Tags

NES Nuclear export signal

Expression

Mammalian

Mutation

The insert comprises only a small sequence of hum…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pMMB67EH-CFP

Plasmid

#90103

Purpose

CFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

CFP

Use

Broad-host range

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_15_UPRpro_X2site

Plasmid

#194427

Purpose

Plasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

UPRpro

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

NA

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_33_PyruPro_X2site

Plasmid

#194429

Purpose

Plasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruPro

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

NA

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge