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pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
NSK021 CMV-TO-p53R248Q (FLP-IN)

Plasmid

#191167

Purpose

Expression of human p53 mutant R248Q in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

p53(R248Q) (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R248Q

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NSK026 CMV-TO-p53R248W (FLP-IN)

Plasmid

#191168

Purpose

Expression of human p53 mutant R248W in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

p53(R248W) (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

R248W

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
H6GB1tevP53SS(1-393)

Plasmid

#200311

Purpose

Bacterial expression of human superstable p53 as His6GB1tev fusion

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (TP53 Human)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

M133L, V203A, N239Y, N268D

Promoter

T7

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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H6SUMO-IntC-P53(304-393)

Plasmid

#200316

Purpose

bacterial expression of P53 TET-CTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Use

Tags

His6-SUMO and designed intein C

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
H6GB1tevP53(1-303)IntN

Plasmid

#200312

Purpose

Bacterial expression of P53 NTAD-DBD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (TP53 Human)

Use

Tags

His6GB1tev and designed intein N

Expression

Bacterial

Mutation

M133L, V203A, N239Y, N268D

Promoter

T7

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lox-stop-Lox p53 R172H

Plasmid

#14854

Depositor

Tyler Jacks

Article

Olive et al Cell. 2004 Dec 17. 119(6):847-60.

Insert

p53 R172H (Trp53 Mouse)

Use

Cre/Lox

Tags

Lox-STOP-lox

Expression

Mammalian

Mutation

Structural mutant R172H. (Murine p53 codon 172 co…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pT5T_hp53_ST

Plasmid

#34922

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 1-393) (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
p53-(1-393)-R273H-N268D

Plasmid

#35087

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R273H-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
Lox-stop-Lox p53 R270H

Plasmid

#14853

Depositor

Tyler Jacks

Article

Olive et al Cell. 2004 Dec 17. 119(6):847-60.

Insert

p53 R270H (Trp53 Mouse)

Use

Cre/Lox

Tags

Lox-STOP-lox

Expression

Mammalian

Mutation

Contact mutant R270H. (Murine p53 codon 270 corre…

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pRRLNeo-pEF1a-p53ash-mCerulean

Plasmid

#69579

Purpose

Expresses p53 tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p53-(1-393)-R175H-N268D

Plasmid

#35086

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R175H-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
PG13-gal (wt p53 binding sites)

Plasmid

#16446

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
MG15-gal (mut p53 binding sites)

Plasmid

#16447

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (mutant) (TP53 )

Use

Beta-gal reporter

Tags

beta-gal

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-73) (72P)

Plasmid

#62083

Purpose

Expression of human p53 (1-73) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72P

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

Plasmid

#69582

Purpose

Expresses p53 tagged with mKate2 and split N-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - N term

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSK-SN3 (wt p53)

Plasmid

#16421

Depositor

Bert Vogelstein

Article

Baker et al Science. 1989 Apr 14. 244(4901):217-21.

Insert

p53 (TP53 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pX62

Plasmid

#46854

Purpose

TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 G245S (TP53 Mouse, Human)

Use

Tags

Expression

Mammalian

Mutation

G245S

Promoter

Available since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Use

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
p53-(1-393)-E285A-N268D

Plasmid

#35090

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - E285A-N268D

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only