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Showing: 181 - 200 of 547 results

  1. pQE30-TEV-pro-conA

    Plasmid
    #159527
    Purpose
    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.
    Depositor
    Joshua Mylne
    Article
    Nonis et al bioRxiv. 2020.
    Insert
    Canavalia ensiformis pro-concanavalin A
    Use
    Tags
    Six-Histidine tag and tobacco etch virus (TEV) pr…
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    159527-plasmid-map-sequence-id-317076

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  2. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

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  4. PYL1_nTEVp

    Plasmid
    #119213
    Purpose
    Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nTEVp in sufion with PYL1
    Use
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119213-plasmid-map-sequence-id-232030

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  5. ABI_cTEVp

    Plasmid
    #119214
    Purpose
    Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cTEVp in fusion with ABI
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119214-plasmid-map-sequence-id-231821

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  9. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  10. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202586-plasmid-map-sequence-id-415635

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  19. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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  20. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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Showing: 181 - 200 of 547 results