-
Plasmid#155358PurposeRifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pSopBWT
Plasmid#112512PurposePsopB driven sopB and sigEDepositorInsertsopB/sigE operon
UseTagsExpressionBacterialMutationPromoterPsopBAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pSB77
Plasmid#19590DepositorInsertthiosulfate reductase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_dGFPmut3-AAV
Plasmid#187377PurposerpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)DepositorInsertdGFPmut3_AAV
UseTagsAAVExpressionBacterialMutationC-terminal AAV tailPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG019 (AVAST type 3)
Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUB11
Plasmid#169699PurposeTemplate plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
UseTags3xFLAG tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNB23
Plasmid#103058Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGpDepositorInsertGFP-OVA
UseTagsExpressionBacterialMutationPromoterhydrogen peroxide-inducible promoter katGpAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB22
Plasmid#103059Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpApDepositorInsertGFP-OVA
UseTagsnoneExpressionBacterialMutationPromoterhmpApAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUB7
Plasmid#169698PurposeTemplate plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU315
Plasmid#169697PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
UseTags1xHA tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSopB2xHA
Plasmid#112513PurposePsopB driven sopB-2xHA and sigEDepositorInsertsopB-2xHA/sigE operon
UseTags2xHAExpressionBacterialMutationSopB C-terminal 2xHAPromoterPsopBAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSU314
Plasmid#169696PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
UseTags1xHA tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU312
Plasmid#169694PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
UseTags1xFLAG tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSU313
Plasmid#169695PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
UseTags1xFLAG tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACCS3-1
Plasmid#78559Purpose4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.DepositorInsertcs3 operon
UseTagsExpressionBacterialMutationPromotertetAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWSK129-stm2585-gp130
Plasmid#174404PurposeBacterial expression of sarA-gp130 chimera under native promoter.DepositorInsertsarA-IL6ST chimera
UseTagsExpressionBacterialMutationPromoterEndogenousAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only