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Showing: 181 - 200 of 304 results

  2. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174644
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Venus-SSPB and iLID-mCherry
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174644-plasmid-map-sequence-id-347042

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  3. pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

    Plasmid
    #122985
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-SspB(milli)-BoNT/B(147-441)
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    SspB contains A58V,R73Q "milli" mutatio…
    Promoter
    human synapsin
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122985-plasmid-map-sequence-id-238429

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  4. pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A)

    Plasmid
    #122986
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-SspB(micro)-BoNT/B(147-441)
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    SspB contains R73Q "micro" mutation, Bo…
    Promoter
    human synapsin
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122986-plasmid-map-sequence-id-238430

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  5. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174645
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
    Use
    Tags
    SSPB(micro)-Venus and iLID-mCherry
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174645-plasmid-map-sequence-id-347047

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  7. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174646
    Purpose
    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…
    Promoter
    CMV (with TetOn)
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174646-plasmid-map-sequence-id-346959

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  8. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174647
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    CMV (with TetOn)
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174647-plasmid-map-sequence-id-345296

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  11. pJK237

    Plasmid
    #73339
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6, Ssp intein, and chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73339-plasmid-map-sequence-id-138207

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  12. pLY071f

    Plasmid
    #227942
    Purpose
    SICLOPPS precursor for cyclo-CRCVFSG
    Depositor
    Tina Wang
    Article
    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038
    Insert
    Evolved Ssp SICLOPPS-CRCVFSG
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Evolved Ssp split inteins with multiple mutations…
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227942-plasmid-map-sequence-id-454431

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  13. pLY071c

    Plasmid
    #227940
    Purpose
    SICLOPPS precursor for cyclo-CHVVGVI
    Depositor
    Tina Wang
    Article
    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038
    Insert
    Evolved Ssp SICLOPPS-CHVVGVI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Evolved Ssp split inteins with multiple mutations…
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    227940-plasmid-map-sequence-id-454446

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  14. pLY071e

    Plasmid
    #227941
    Purpose
    SICLOPPS precursor for cyclo-CDLGVFR
    Depositor
    Tina Wang
    Article
    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038
    Insert
    Evolved Ssp SICLOPPS-CDLGVFR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Evolved Ssp split inteins with multiple mutations…
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227941-plasmid-map-sequence-id-454433

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  15. pLY090a

    Plasmid
    #227938
    Purpose
    SICLOPPS precursor for cyclo-CRVWCAR
    Depositor
    Tina Wang
    Article
    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038
    Insert
    Evolved Ssp SICLOPPS-CRVWCAR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Evolved Ssp split inteins with multiple mutations…
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227938-plasmid-map-sequence-id-454432

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  16. pLY090b

    Plasmid
    #227939
    Purpose
    SICLOPPS precursor for cyclo-CRVYQVL
    Depositor
    Tina Wang
    Article
    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038
    Insert
    Evolved Ssp SICLOPPS-CRVYQVL
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Evolved Ssp split inteins with multiple mutations…
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227939-plasmid-map-sequence-id-454430

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Showing: 181 - 200 of 304 results