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Showing: 181 - 200 of 3797 results

  3. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Use
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    IRES and tac
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pSBbi-Pur/GDI1-splitTurboID

    Plasmid
    #198219
    Purpose
    Transposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    GID1
    Use
    Transposon
    Tags
    5’ part of TurboID, up to E256
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198219-plasmid-map-sequence-id-389399

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  13. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD and PLtetO
    Available since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

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  14. pCoHygro L16_shRNA of spliced Copia

    Plasmid
    #71389
    Purpose
    knock down expression of spliced Copia in Drosophila melanogaster
    Depositor
    Timothy Nilsen
    Insert
    Copia
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6
    Available since
    Nov. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    71389-plasmid-map-sequence-id-129772

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  16. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

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  17. Spike Display_B.1.427/B.1.429

    Plasmid
    #172738
    Purpose
    Expression vector for SARS-CoV-2 (B.1.427/B.1.429) spike used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S B.1.427/B.1.429 (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 8, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172738-plasmid-map-sequence-id-339247

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  20. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

    Plasmid
    #184334
    Purpose
    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
    Depositor
    Peter Scheiffele
    Article
    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0
    Insert
    Nrxn3-alpha (Nrxn3 Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    184334-plasmid-map-sequence-id-365498

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Showing: 181 - 200 of 3797 results