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pFS_0374_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1_RC

Plasmid

#116986

Purpose

Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Teredinibacter turnerae T8412 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0372_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2_RC

Plasmid

#116984

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0373_pET30-RT-CRISPR_Teredinibacter_turnerae_T8412_ARRAY_1

Plasmid

#116985

Purpose

Expresses TtRT-Cas1-Cas2 under pT7lac promoter, encodes TtCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Teredinibacter turnerae T8412 RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0370_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1_RC

Plasmid

#116982

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0371_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2

Plasmid

#116983

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUT1192-HQ858812

Plasmid

#27894

Depositor

John Gray, Erich Grotewold

Article

Yilmaz et al Plant Physiol. 2009 Jan;149(1):171-80. d

Insert

PLATZ transcription factor encoded by Os02g0692700 LOC_Os02g46610

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pIVT-eeBxb1

Plasmid

#232748

Purpose

IVT template for making modRNA encoding eeBxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Expression

Mutation

V74A, E229K, V375I

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Flp-2A-BleoR

Plasmid

#232752

Purpose

IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Inserts

FlpO
BleoR

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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TCARZ-CBh-GFP-SV40PA-GF3-R4A22

Plasmid

#203767

Purpose

For production of template RNA for use in PRINT, GFP under CBh promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

CBh

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TCARZ-CMV-GFP-PAmin-GF3-R4A22

Plasmid

#203768

Purpose

For production of template RNA for use in PRINT, GFP under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAS088

Plasmid

#211502

Purpose

AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Type

Empty backbone

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCE046-SiT-Cas12a-[Repr]

Plasmid

#128132

Purpose

Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Repr]-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCE059-SiT-Cas12a-[Activ]

Plasmid

#128136

Purpose

Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Activ]-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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TCARZ-SV40-GFP-PAmin-GF3-R4A22

Plasmid

#203769

Purpose

For production of template RNA for use in PRINT, GFP under SV40 promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Tags

Expression

Mutation

Promoter

SV40

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TCARZ-CMV-Cherry-PAmin-GF3-R4A22

Plasmid

#203770

Purpose

For production of template RNA for use in PRINT, mCherry under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for mCherry

Use

Ivt template

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCI108-SiT-Cas12a-[Cond]

Plasmid

#128407

Purpose

Conditionally express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS006

Plasmid

#211506

Purpose

AAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequences

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Type

Empty backbone

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI118-SiT-Cas12a-[Ind]

Plasmid

#128406

Purpose

Inducible expression of both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXint129

Plasmid

#178208

Purpose

Encodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.

Depositor

Gregory Phillips

Article

Platt et al Plasmid. 2000 Jan . 43(1):12-23.

Insert

xis excisionase, int integrase (xis, lambdap32 Bacteriophage lambda)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacI

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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