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Showing: 181 - 200 of 1037 results

  1. pJK547

    Plasmid
    #72449
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Val319 to Phe
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72449-plasmid-map-sequence-id-133874

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  2. pJK550

    Plasmid
    #72450
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted Val2
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72450-plasmid-map-sequence-id-133877

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  3. pJK323

    Plasmid
    #73495
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73495-plasmid-map-sequence-id-135755

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  4. pJK322

    Plasmid
    #73494
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    n/a
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73494-plasmid-map-sequence-id-135746

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  5. pJK553

    Plasmid
    #72452
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72452-plasmid-map-sequence-id-133889

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  6. pJK636

    Plasmid
    #72466
    Purpose
    Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72466-plasmid-map-sequence-id-133767

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  8. pJK582

    Plasmid
    #71618
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala mutant (AaPurE1-S57A)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to alanine, additional N-termin…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71618-plasmid-map-sequence-id-131633

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  9. pJK583

    Plasmid
    #71619
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys mutant (AaPurE1-S57C)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to cysteine, additional N-termi…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71619-plasmid-map-sequence-id-131637

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  10. pJK584

    Plasmid
    #71620
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr mutant (AaPurE1-S57T)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to threonine, additional N-term…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71620-plasmid-map-sequence-id-131638

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  11. pJK608

    Plasmid
    #71621
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp mutant (AaPurE1-S57D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to aspartate, additional N-term…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71621-plasmid-map-sequence-id-131639

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  12. pJK677

    Plasmid
    #71623
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val mutant (AaPurE1-S57V)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to valine, additional N-termina…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71623-plasmid-map-sequence-id-131640

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  13. pJK686

    Plasmid
    #71624
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asn mutant (AaPurE1-S57N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes serine-57 to asparagine, additional N-ter…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71624-plasmid-map-sequence-id-131641

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  14. pJK538

    Plasmid
    #72445
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72445-plasmid-map-sequence-id-133868

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  15. pJK505

    Plasmid
    #71594
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, additiona…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71594-plasmid-map-sequence-id-131669

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  16. pJK506

    Plasmid
    #71596
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-165 to phenylalanine, addition…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71596-plasmid-map-sequence-id-131670

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  20. pJK626

    Plasmid
    #72461
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to cysteine, tyrosine-…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72461-plasmid-map-sequence-id-133899

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Showing: 181 - 200 of 1037 results