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Showing: 181 - 200 of 201 results

  6. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197064
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197064-plasmid-map-sequence-id-402605

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  15. pCR-Hyg

    Plasmid
    #158708
    Purpose
    For cloning and constitutive expression of crRNAs in mycobacterium
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    crRNA
    Use
    CRISPR and Synthetic Biology; Mycobacteria expres…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Hsp60
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158708-plasmid-map-sequence-id-320872

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  16. pCR-Zeo

    Plasmid
    #158709
    Purpose
    For cloning and constitutive expression of crRNAs in mycobacterium
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    crRNA
    Use
    CRISPR and Synthetic Biology; Mycobacteria expres…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Hsp60
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158709-plasmid-map-sequence-id-320874

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  17. pJV53-Cas12a

    Plasmid
    #158706
    Purpose
    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158706-plasmid-map-sequence-id-320865

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  18. pMS1088

    Plasmid
    #80159
    Purpose
    pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme
    Depositor
    Marcelo Sousa
    Article
    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/
    Insert
    HisSUMO-Cys-4xNuG2-Ald
    Use
    Tags
    HisSUMO
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    80159-plasmid-map-sequence-id-150741

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  19. pMS1251

    Plasmid
    #80163
    Purpose
    pET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme
    Depositor
    Marcelo Sousa
    Article
    Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/
    Insert
    HisSUMO-Ald-4xNuG2-Ald
    Use
    Tags
    HisSUMO
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    80163-plasmid-map-sequence-id-150737

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Showing: 181 - 200 of 201 results