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pcDNA3-Lyn-GR-AKAR3

Plasmid

#173016

Purpose

Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Lyn-GR-AKAR3

Use

Tags

EGFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMAZ-SK

Plasmid

#73962

Purpose

self-killing gRNA plasmid pMAZ-SK

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Insert

gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB

Plasmid

#11777

Depositor

Didier Trono, Patrick Aebischer

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLVU-tTR-KRAB

Plasmid

#11645

Depositor

Didier Trono, Patrick Aebischer

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, no tetO

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pJEC779

Plasmid

#234348

Purpose

Modular RENDR, GFP output, targets synthetic target

Depositor

James Chappell

Article

Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

Insert

Modular RENDR, GFP output

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PJ23119

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pJEC780

Plasmid

#234349

Purpose

Expressed synthetic RNA target for RENDR

Depositor

James Chappell

Article

Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

Insert

Synthetic RNA target for RENDR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PJ23119

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pDS3-X4

Plasmid

#70140

Purpose

Yeast chr. integration vector with DS3 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS3

Use

Tags

Expression

Yeast

Mutation

Promoter

AgTEF1

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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FRB-TagRFPT-CAAX

Plasmid

#87710

Purpose

FRB-tagged TagRFPT; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FRB-TagRFPT-CAAX

Use

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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FKBP-Dronpa

Plasmid

#87709

Purpose

FKBP-tagged Dronpa.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FKBP-Dronpa

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Dronpa-DEVD-TagRFPT

Plasmid

#87704

Purpose

Fluorescent biosensor for super-resolution imaging of caspase activity.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

Dronpa-DEVD-TagRFPT

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Dronpa-FHA1

Plasmid

#87711

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

Dronpa-FHA1

Use

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA7CR_2.0

Plasmid

#73950

Purpose

pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

Depositor

Alex Nielsen

Article

Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

Inserts

cas9
lambda beta
dam
recX

Use

Tags

Expression

Bacterial

Mutation

Promoter

pAra and pTet

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mito

Plasmid

#71542

Purpose

Biomedical Research

Depositor

David Sidransky

Article

Dasgupta et al Cancer Res. 2008 Feb 1;68(3):700-6. doi:

Insert

myc-mito

Use

Cytomegalovirus

Tags

none

Expression

Mutation

Promoter

CMV

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
pDS2-aurZ

Plasmid

#70151

Purpose

Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurZ

Use

Tags

Expression

Yeast

Mutation

Promoter

Tef

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLVPT-tTR-KRAB

Plasmid

#11642

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Didier Trono, Patrick Aebischer

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLVPRT-tTR-KRAB

Plasmid

#11648

Purpose

Tet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Didier Trono, Patrick Aebischer

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hPrion, GFP, tTR-KRAB, Tet-on

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-3xflagGREB1c

Plasmid

#112223

Purpose

mammalian expression of flag tagged GREB1c

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1c

Use

Tags

3x flag

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-1 Sesn3

Plasmid

#61875

Purpose

bacterial expression of a GST-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

none

Promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Flag-Sesn3

Plasmid

#61869

Purpose

Transient overexpression of Flag-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pDS1U-PKS12

Plasmid

#70150

Purpose

Fusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

PKS12 (PKS12 Fusarium graminearum)

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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