Addgene

We narrowed to 2,331 results for: GCG

Showing: 181 - 200 of 2331 results

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pBB75-ncRNA

Plasmid

#194087

Purpose

Expresses Ec86 ncRNA in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 ncRNA

Use

Tags

Expression

Mutation

Promoter

T7

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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1161F

Plasmid

#183138

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTX208

Plasmid

#89268

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsYSA-gRNA01 and OsYSA-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Maize Ubiquitin1 promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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1114H

Plasmid

#200640

Purpose

An. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile males

Depositor

Omar Akbari

Article

Smidler et al bioRxiv [Preprint]. 2023 Jul 21:2023.07.

Insert

gRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS2

Plasmid

#174302

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pW318-lenti-sg2-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189948

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg2-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW319-lenti-sg3-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189949

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg3-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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1056K

Plasmid

#183137

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide

Plasmid

#121993

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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gh104

Plasmid

#106774

Purpose

expression of gRNA targeting XXYLT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

XXYLT1 (XXYLT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rssA

Plasmid

#89957

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rssA gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCS253

Plasmid

#33209

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

RAP1A (RAP1A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aCgCgaa, aCgCgaa

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCS260

Plasmid

#33211

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

SYNM (SYNM Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aCgCgaa, aaCgCgaa

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCS249

Plasmid

#33203

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

WBP4 3'UTR (WBP4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aCgCgaa, aCgCgaa

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCS250

Plasmid

#33205

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

NEK6 3'UTR (NEK6 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aaCgCgaa, aaCgCgaa

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
Circular 100,50 (RAB7A)

Plasmid

#170117

Purpose

AAV vector carrying a guide RNA targeting the human RAB7A mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 100,50 guide RNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE unc13a GFP KI

Plasmid

#131498

Purpose

Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Unc13a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #1 GFP

Plasmid

#106950

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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PLK1 G6.1 gRNA

Plasmid

#90835

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PLK1 (Guide Designation G6.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_1

Plasmid

#73533

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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