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Plasmid#161552PurposeConstitutive expression of intC-ZF10-NLS-HA under the EF1α promoterDepositorInsertintC-ZF10-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM505 EF1α 3xFLAG-NLS-DsRed-Express2-ZF1 in TUPV2
Plasmid#161535PurposeConstitutive expression of 3xFLAG-NLS-DsRed-Express2-ZF1 under the EF1α promoterDepositorInsert3xFLAG-NLS-DsRed-Express2-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM506 EF1α 3xFLAG-NLS-DsDed-ZF1 in TUPV2
Plasmid#161536PurposeConstitutive expression of 3xFLAG-NLS-DsDed-ZF1 gene under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM512 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2
Plasmid#161538PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM520 EF1α 3xFLAG-NLS-VP64-ZF10 in TUPV4
Plasmid#161539PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF10 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF10
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM574 ZF2x6-C 3xFLAG-NLS-DsDed-ZF1 in TUPV2
Plasmid#161541PurposeInducible expression of 3xFLAG-NLS-DsDed-ZF1 under the ZF2x6-C promoterDepositorInsert3xFLAG-NLS-DsDed-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF2x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-Bxb1-LP-v2-TC
Plasmid#194327PurposeCLYBL targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-v2-TC
Plasmid#194326PurposeAAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.DepositorInsertloxP-EBFP2-attP-BleoR-lox251
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-I-PhoCl-HCVp
Plasmid#87697PurposeExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cellsDepositorInsertI-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
UseTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-941
Plasmid#103757PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-941 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJM592 EF1α 3xFLAG-NLS-DsDed-ZF10-intN-VP64 in TUPV2
Plasmid#161568PurposeConstitutive expression of 3xFLAG-NLS-DsDed-ZF10-intN-VP64 under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-ZF10-intN-VP64
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM595 ZF6x6-C intC-ZF1-ZF2-NLS-HA in TUPV2
Plasmid#161569PurposeInducible expression of intC-ZF1-ZF2-NLS-HA under the ZF6x6-C promoterDepositorInsertintC-ZF1-ZF2-NLS-HA
UseSynthetic BiologyTagsHAExpressionMammalianMutationPromoterZF6x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM553 ZF10x6-C 3xFLAG-NLS-DsDed-ZF1-PEST in TUPV2
Plasmid#161540PurposeInducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoterDepositorInsert3xFLAG-NLS-DsRed-ZF1-PEST
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF10x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTW087
Plasmid#115935PurposeORF insert for replicon MoCloDepositorInsertLvl0 EBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-1
Plasmid#103160PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-1 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-1 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG::Nucbow
Plasmid#158996PurposeMulticolor nuclear-restricted labeling with a genome-integrating piggyBac transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
UseTagsAll FPs are fused to H2BExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29b-3p
Plasmid#103394PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29b-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133b
Plasmid#103223PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133b target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133b target (MIR133B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CaTCH BC1
Plasmid#154195PurposeLentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)DepositorInsertCaTCH BC1
UseLentiviral; Catch barcode labelingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only