Addgene

We narrowed to 827 results for: nanobodies

Showing: 361 - 400 of 827 results

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pET21-pelB-LaMIgG-9

Plasmid

#228993

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-9

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-1

Plasmid

#228974

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-1

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaRIgG-7

Plasmid

#228997

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-7

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-44

Plasmid

#228981

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-44

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-45

Plasmid

#228982

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-45

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-8

Plasmid

#228992

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-8

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-10

Plasmid

#228978

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-10

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaRIgG-1

Plasmid

#228995

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-1

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-8

Plasmid

#228990

Purpose

For bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-8

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-4

Plasmid

#228964

Purpose

For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-4

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-5

Plasmid

#228991

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-5

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-5

Plasmid

#228987

Purpose

For bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-5

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-6

Plasmid

#228988

Purpose

For bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-6

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-8

Plasmid

#228976

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-8

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAK461

Plasmid

#140699

Purpose

NBαRb-IgG-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαRb-IgG-Fc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMAK462

Plasmid

#140700

Purpose

NBαMs/Rt-IgG1-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs/Rt-IgG1-Fc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_mNeonGreen

Plasmid

#209425

Purpose

Express mNeonGreen in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

mNeonGreen

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GNb-RNb

Plasmid

#128787

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-RNb

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RNb-FKBP

Plasmid

#128779

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GNb-FKBP

Plasmid

#128771

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-FKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GNb-mCherry

Plasmid

#128769

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GNb-pHuji

Plasmid

#128776

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-pHuji

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GNb-SNAPf

Plasmid

#128770

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RNb-GFP

Plasmid

#128777

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ssGNb-mCherry

Plasmid

#128788

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

ssGNb-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RNb-SNAPf

Plasmid

#128778

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-SNAPf

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RNb-FRB

Plasmid

#128780

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GNb-FRB

Plasmid

#128772

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-FRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RNb-zdk1

Plasmid

#128781

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-zdk1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RNb-SEpHluorin

Plasmid

#128785

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-SEpHluorin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ssRNb-GFP

Plasmid

#128791

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

ssRNb-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-26b-Nb127D01-HA-His

Plasmid

#171566

Purpose

Bacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-HA-His (CXCR2 Human)

Use

Tags

HA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Use

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
(271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

Plasmid

#168102

Purpose

HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nEPEA-(EAAAK)3-OGA(544-706)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

OGA truncated to contain residues 544-706

Promoter

T7/CMV

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-26b-NbVHH05-ALFA-His

Plasmid

#171569

Purpose

Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-ALFA-His (UBE2J1 Human)

Use

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT-HGP-v3-NbVHH05-hIgG

Plasmid

#171565

Purpose

Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)

Use

Tags

BiP signal peptide and hIgG1-hinge-N297A-TEV site…

Expression

Insect

Mutation

Promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-LaG16-APT1

Plasmid

#234423

Purpose

Express APT1 with the anti-GFP nanobody LaG16 fused at the N terminus

Depositor

William Fuller

Article

Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

Insert

APT1 fused to LaG16 (LYPLA1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-LaG16-APT2

Plasmid

#234422

Purpose

Express APT2 with the anti-GFP nanobody LaG16 fused at the N terminus

Depositor

William Fuller

Article

Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

Insert

APT2 fused to LaG16 (LYPLA2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 361 - 400 of 827 results

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