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pNCMV 16E6no* I128T
Plasmid#47706PurposeDefective for E6AP bindingDepositorInsertHPV 16 E6no* (E6 HPV)
UseTagsFLAG and HAExpressionMammalianMutationdel first 7 aa, I128T, V42L*PromoterCMVAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMT440
Plasmid#8609DepositorInsertbarnase, barstar
UseTagsas pMT416ExpressionBacterialMutationV36 codon of barnase of pMT416 replaced by polyli…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCSJ95
Plasmid#104343PurposeExpress yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertFBP1
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSJ125
Plasmid#104344PurposeExpress yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertMDH2
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCMV 16E6no* Y54D
Plasmid#47705PurposeDefective for p53 bindingDepositorInsertHPV 16 E6no* (E6 HPV)
UseTagsFLAG and HAExpressionMammalianMutationdel first 7 aa,Y54D, V42L*PromoterCMVAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiN 18E6no*
Plasmid#37446PurposeLentiviral expression of HPV 18E6no* in mammalian cellsDepositorInsertHPV 18E6no* (E6 HPV)
UseLentiviralTagsFlag and HAExpressionMammalianMutationV44APromoterCMVAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSF503
Plasmid#222587PurposeBB3 gsdA tet-on overexpression cassette with homologous flanks for integration at pyrG locusDepositorArticleInsertgsdA (gsdA )
UseA. nigerTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insert,…Promotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF522
Plasmid#222588PurposeBB3 ZWF1 tet-on overexpression cassette with homologous flanks for integration at pyrG locusDepositorArticleInsertZWF1
UseA. nigerTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF8
Plasmid#222592PurposeBB2 gsdA tet-on overexpression cassetteDepositorArticleInsertgsdA
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insert,…Promotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSECB sgCASP9
Plasmid#211525PurposeDeletes CASP9DepositorInsertsgCASP9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK1520 sgFADD
Plasmid#211527PurposeDeletes FADDDepositorInsertsgPIDD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgUCKL1
Plasmid#211523PurposeDeletes UCKL1DepositorInsertsgUCKL1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgUCK2
Plasmid#211522PurposeDeletes UCK2DepositorInsertsgUCK2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgUGP2
Plasmid#211521PurposeDeletes UGP2DepositorInsertsgUGP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgBAK1
Plasmid#211520PurposeDeletes BAK1DepositorInsertsgBAK1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v2 sgCASP8
Plasmid#211519PurposeDeletes CASP8DepositorInsertsgCASP8
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
TalO8-HSP30-CFP
Plasmid#178304PurposeCoTrIP plasmid with HSP30 promoter driving CFP's expressionDepositorInsertHSP30pr-CFP-tAdh1
UseTagsExpressionYeastMutationPromoterHSP30 and NAAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TalO8-HSP26-CFP
Plasmid#178306PurposeCoTrIP plasmid with HSP26 promoter driving CFP's expressionDepositorInsertHSP26pr-CFP-tAdh1
UseTagsExpressionYeastMutationPromoterHSP26 and NAAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TalO8-GLC3-CFP
Plasmid#178307PurposeCoTrIP plasmid with GLC3 promoter driving CFP's expressionDepositorInsertGlc3pr-CFP-tAdh1
UseTagsExpressionYeastMutationPromoterGLC3 and NAAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSJ182
Plasmid#159723PurposeExpress Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCgDepositorInsertGID4 (VID24 Budding Yeast)
UseYeast two-hybrid assayTags3xflag and Gal4ADExpressionYeastMutationremove stop codonPromoterADH1Available sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-LGK
Plasmid#81165PurposepETconNK with levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
UseTagsc-mycExpressionBacterial and YeastMutationPromoterGAL1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAF259
Plasmid#105494PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOptDepositorInsertBitlucopt
UseAtc-dependent expression in c. difficileTagsExpressionMutationPromoterPtetAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
E228Q Vps4A-HA
Plasmid#200087Purpose3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vectorDepositorInsertVPS4A (VPS4A Human)
UseLentiviralTags3xHAExpressionMammalianMutationE228QPromoterCMVAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromotersgkAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-citrate
Plasmid#225251PurposeBacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrateDepositorInsertqmTQ2-citrate
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only
