Skip to main content
Addgene
Showing: 181 - 200 of 2410 results

  1. pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)

    Plasmid
    #139991
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139991-plasmid-map-sequence-id-275523

    Enlarge

  2. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid
    #139993
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139993-plasmid-map-sequence-id-275508

    Enlarge

  6. pLenti-CMV-tdTomato-miRE_shRen-Puro

    Plasmid
    #135687
    Purpose
    Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135687-plasmid-map-sequence-id-327656

    Enlarge

  7. pLenti-CMV-GFP-miRE_shRen-Blast

    Plasmid
    #135684
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135684-plasmid-map-sequence-id-327610

    Enlarge

  8. pLenti-CMV-tdTomato-miRE_shRen-Blast

    Plasmid
    #135686
    Purpose
    Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135686-plasmid-map-sequence-id-327617

    Enlarge

  9. pLenti-CMV-GFP-miRE_shTHUMPD3-Blast

    Plasmid
    #135690
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shRosa26
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135690-plasmid-map-sequence-id-327708

    Enlarge

  10. pLenti-CMV-GFP-miRE_shTHUMPD3-Puro

    Plasmid
    #135691
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRosa26
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135691-plasmid-map-sequence-id-327716

    Enlarge

  11. pLenti-CMV-GFP-miRE_shSCR-Puro

    Plasmid
    #135689
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shScrambled
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135689-plasmid-map-sequence-id-327714

    Enlarge

  12. pLenti-CMV-GFP-miRE_shSCR-Blast

    Plasmid
    #135688
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selection
    Depositor
    Florian Karreth
    Insert
    shScrambled
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135688-plasmid-map-sequence-id-327712

    Enlarge

  13. pLenti-CMV-GFP-miRE_shRen-Puro

    Plasmid
    #135685
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135685-plasmid-map-sequence-id-327612

    Enlarge

  14. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid
    #139989
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139989-plasmid-map-sequence-id-275392

    Enlarge

  16. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid
    #208967
    Purpose
    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208967-plasmid-map-sequence-id-415814

    Enlarge

  17. KI(GAPDH)-P2A-EGFP-CMV-NeoR

    Plasmid
    #114009
    Purpose
    HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection
    Depositor
    Pavel Volchkov
    Article
    Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126
    Inserts
    GAPDH left arm (GAPDH Chicken)
    eGFP
    NeoR
    GAPDH right arm
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    -, CMV, and endogenous promoter (chicken GAPDH in…
    Available since
    Jan. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    114009-plasmid-map-sequence-id-261238

    Enlarge

  20. pAAV-CMV-rtTA-IRES-GFP-WPRE-pA

    Plasmid
    #183709
    Purpose
    AAV designed to express rtTA and eGFP from a CMV promoter
    Depositor
    Jonathan Ploski
    Insert
    rtTA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183709-plasmid-map-sequence-id-360828

    Enlarge

Showing: 181 - 200 of 2410 results