Addgene

We narrowed to 357 results for: gag pol

Showing: 91 - 100 of 357 results

Results per page:

Sort results by:

pCfb13215

Plasmid

#219890

Purpose

The base plasmid of TUNEYALI for TF25

Depositor

Irina Borodina

Insert

Contains gRNA targeting TF25 (YALI1_B18134g) and homologous arm matching TF25

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGO186N-KS1

Plasmid

#174301

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGO186N-KS2

Plasmid

#174302

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLTE EGFP

Plasmid

#67044

Purpose

Cell-free expression control

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EGFP

Use

Cell-free expression

Tags

Myc

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G05 sfGFP

Plasmid

#67045

Purpose

Cell-free expression control

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

sfGFP

Use

Cell-free expression

Tags

mCherry

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
L2_NOP1gRNA-Cas9-CsA

Plasmid

#136139

Purpose

Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L2_lacZgRNA-Cas9-CsA

Plasmid

#136138

Purpose

Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 PC

Plasmid

#67132

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PC (PC Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only