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Plasmid#214727PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Quartet (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet_NoLinker
Plasmid#214731PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTagDepositorInsertSpyTag-Quartet_NoLinker (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP786_SpyCatcher-EZH2-FL-CO
Plasmid#211779PurposeSpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-KRAB
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-DIIG
Plasmid#201196PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-DIIG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-SWG
Plasmid#201194PurposeExpresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-SWG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-PEED
Plasmid#201195PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-PEED
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NDV-HN-SpyTag
Plasmid#160000PurposeExpresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cellsDepositorInsertNDV-HN-SpyTag
UseTagsHis6-tagExpressionMammalianMutationPromoterChicken beta-actin promoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B4.SPy1128
Plasmid#11531DepositorInsertphosphotransacetylase
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only