-
Plasmid#183327PurposeAll-in-One CRISPRko system with a guide RNA that targets VEGFA geneDepositorInsertVEGFA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-VEGFA121
Plasmid#73017PurposeRetroviral expression of human VEGFA121DepositorInsertVEGFA (VEGFA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
py100-sgRNA_hVEGFA
Plasmid#198282PurposeExpression of SpCas9 guide RNA specific for human VEGFA locusDepositorInsertpy100-sgRNA_hVEGFA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-VEGFA
Plasmid#140588PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorInsertgRNA VEGFA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_SaCas9_sgRNA2
Plasmid#107330PurposeU6 driven SaCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_SpCas9
Plasmid#107328PurposeU6 driven SpCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBluescript_VEGFA-TS3_NmCas9
Plasmid#107329PurposeU6 driven NmCas9 sgRNA expression for VEGFA site 3DepositorInsertVEGFA-TS3 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-hVEGFA
Plasmid#112553Purpose3'UTR reporter assayDepositorInsertVEGFA (VEGFA Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA_VEGFA-mcherry
Plasmid#159790PurposeS. pyogenes sgRNA collocated with pegRNA targeting human VEGFA geneDepositorInsertspacer of sgRNA targeting VEGFA gene (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRE-VEGFA EF1a-rtTA3
Plasmid#195881PurposeLentiviral construct of doxycycline inducible VEGFADepositorInsertVEGFA (Vegfa Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM116: CasRx VEGFA presgRNA
Plasmid#166868PurposeU6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.DepositorInsertHuman VEGFA targeting presgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM117: VEGFA array presgRNA
Plasmid#166869PurposeU6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.DepositorInsertHuman VEGFA targeting array presgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 VEGFA-TSS-guide4
Plasmid#125502PurposeCRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 VEGFA-TSS-guide2
Plasmid#125501PurposeCRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 VEGFA-TSS-guide1
Plasmid#125500PurposeCRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS6-ZF(VEGFA)-StaPL(AI)-YFP-VPR
Plasmid#111502PurposeExpresses a zinc finger specific to the human VEGFA locus, which is linked to a VPR transcriptional activation domain via a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].DepositorInsertZF(VEGFA)-StaPL(AI)-YFP-VPR
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
Plasmid#111504PurposeExpresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].DepositorInsertZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, S122R, a…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE
Plasmid#89609Purposeover-expression of VEGF-A(165) in human cellsDepositorInsertVEGF-A(165) (VEGFA Human)
UseLentiviralTagsExpressionMammalianMutationK188EPromoterMNDU3Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM124: pAAV-EFS-CasRx-VEGFA presgRNA
Plasmid#166872PurposeAAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editingDepositorInsertsU6-VEGFA presgRNA
RfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianMutationPromoterEFS and U6Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM125: pAAV-EFS-CasRx-VEGFA array presgRNA
Plasmid#166873PurposeAAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editingDepositorInsertsU6-VEGFA array presgRNA
RfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianMutationPromoterEFS and U6Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only