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Plasmid#161810Purposeguide RNA for Upf1DepositorInsertsgRNA targeting mouse Upf1 (Upf1 Synthetic, Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pci-EGFP-UPF1-WT
Plasmid#135994PurposeWT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF1-3'UTR
Plasmid#136036PurposeUPF1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (TTATTACCCAGAATAAGATGC)DepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF1-CDS
Plasmid#136037PurposeUPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)DepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-DrUPF1_F
Plasmid#146281PurposeBacterial Expression of DrUPF1DepositorInsertDrUPF1 (upf1 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1WT (pRKB264)
Plasmid#99146PurposeInducible lentiviral construct to express FLAG-tagged UPF1wtDepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-siRNAres_P
Plasmid#147196PurposeMammalian Expression of HsUpf1-siRNAresDepositorInsertHsUpf1-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-TO-EGFP-UPF1-WT
Plasmid#136000PurposeDOX-inducible WT UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pci-EGFP-UPF1-DEAA
Plasmid#135996PurposeDEAA UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationDE647AAPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-lambdaN-UPF1-WT
Plasmid#136006PurposeWT UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)DepositorInsertUPF1
UseTagsLambdaNExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pci-EGFP-UPF1-R615A
Plasmid#135995PurposeR615A UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cellsDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationR615APromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmUPF1-V5His6_E
Plasmid#146165PurposeInsect Expression of DmUPF1DepositorInsertDmUPF1 (Upf1 Fly)
UseTagsExpressionInsectMutationtwo non silent mutations E531G and G995E compared…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1_1-925-siRNAres_P
Plasmid#147191PurposeMammalian Expression of HsUpf1_1-925-siRNAresDepositorInsertHsUpf1_1-925-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation4 non silent mutations A69S, E167G, K282R and A4…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-del296-926_P
Plasmid#147195PurposeMammalian Expression of HsUpf1-del296-926DepositorInsertHsUpf1-del296-926 (UPF1 Human)
UseTagsExpressionMammalianMutation4 non silent mutations A69S, E167G, K282R and T1…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1_115-1129-siRNAres_P
Plasmid#147193PurposeMammalian Expression of HsUpf1_115-1129-siRNAresDepositorInsertHsUpf1_115-1129-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…PromoterAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-S1107A-siRNAres_S
Plasmid#147478PurposeMammalian Expression of HsUpf1-S1107A-siRNAresDepositorInsertHsUpf1-S1107A-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-V161ER162E-siRNAres_Q
Plasmid#147332PurposeMammalian Expression of HsUpf1-V161ER162EDepositorInsertHsUpf1-V161ER162E (UPF1 Human)
UseTagsExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1_73-1129-siRNAres_P
Plasmid#147192PurposeMammalian Expression of HsUpf1_73-1129-siRNAresDepositorInsertHsUpf1_73-1129-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-V206E-siRNAres_P
Plasmid#147197PurposeMammalian Expression of HsUpf1-V206E-siRNAresDepositorInsertHsUpf1-V206E-siRNAres (UPF1 Human)
UseTagsExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmUPF1-siRNAres-V5His6_E
Plasmid#146168PurposeInsect Expression of DmUPF1-siRNAresDepositorInsertDmUPF1-siRNAres (Upf1 Fly)
UseTagsExpressionInsectMutation1 non silent mutations: G995E compared to the seq…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only