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LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

Plasmid

#170255

Purpose

Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

SV40 Large T Antigen

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SV40 1: pBSSVD2005

Plasmid

#21826

Depositor

David Ron

Insert

SV40 Large T (SV40gp6 simian virus)

Use

Tags

Expression

Mammalian

Mutation

likely expresses aa109-708 of the large T antigen

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Ef1a_Large T-antigen_Ires_Puro

Plasmid

#18922

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

Large T Antigen (SV40gp6 Simian virus 40)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pET28a SV40 ST full length

Plasmid

#132636

Purpose

expresses SV40 ST full length in E. coli

Depositor

Wenqing Xu

Article

Cho et al PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1

Insert

SV40 small t antigen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetOminiCMV-SV40LT

Plasmid

#136616

Purpose

Dox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cells

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

SV40 large T antigen

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5 E107K T Ag

Plasmid

#133710

Purpose

Large T antigen mutant form in pSG5 vector for mammalian expression.

Depositor

James A. DeCaprio

Article

Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

Insert

SV40gp6 (SV40gp6 SV40)

Use

Tags

Expression

Mammalian

Mutation

E107K

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG small T 110

Plasmid

#9061

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 110 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

contains only the N-termial 110 amino acids

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pMIG small T H42Q

Plasmid

#9062

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen H42Q (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

H42Q. Renders J domain nonfunctional.

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pMIG small T 88

Plasmid

#10691

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 88 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

contains only the N-termial 88 amino acids

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T 110

Plasmid

#9063

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 110 (SV40gp7 simian virus)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Contains only the N-termial 110 amino acids

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pMIG small T C97S E102Q

Plasmid

#9060

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 97, 102 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

C97S, E102Q

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T 88

Plasmid

#10690

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 88 (SV40gp7 simian virus)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Contains only the N-termial 88 amino acids

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T C97S E102Q

Plasmid

#9064

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 97, 102 (SV40gp7 simian virus)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

C97S, E102Q

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pMIG small T

Plasmid

#9059

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pBABE-puro SV40 LT

Plasmid

#13970

Depositor

Thomas Roberts

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

SV40 LT (SV40gp6 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-zeo small T H42Q

Plasmid

#9065

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pRSVBneoT

Plasmid

#22883

Depositor

James Pipas

Article

Srinivasan et al Mol Cell Biol. 1997 Aug . 17(8):4761-73.

Insert

Early region SV40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pBABE-neo largeTcDNA

Plasmid

#1780

Purpose

Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-zeo smallT

Plasmid

#8583

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only