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pRS426-TUB1

Plasmid

#60385

Purpose

Expression of yeast alpha tubulin (TUB1) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS426-ACT_stable

Plasmid

#127609

Purpose

high-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Stable ACT1 intron (Mut)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-ACT_unstable

Plasmid

#127608

Purpose

high-copy URA-selectable plasmid, WT ACT1 intron (unstable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Unstable ACT1 intron (WT)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-ECM_stable

Plasmid

#127606

Purpose

high-copy URA-selectable plasmid, WT ECM33 intron (stable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Stable ECM33 intron (WT)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426-HsFECH

Plasmid

#174524

Purpose

Expresses human ferrochelatase (FECH) in yeast

Depositor

Oleh Khalimonchuk

Article

Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

Insert

FECH with yeast HEM15 promoter and terminator (FECH Human, Synthetic, Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Lacks original mitochondrial targeting sequence (…

Promoter

HEM15 (yeast, 257 bp)

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-ECM_unstable

Plasmid

#127607

Purpose

high-copy URA-selectable plasmid, Mut ECM33 intron (unstable) inserted into URA3

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

Unstable ECM33 intron (Mut)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426GFP-2×PH(PLCδ)

Plasmid

#36092

Depositor

Scott Emr

Article

Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

Insert

2xPH(PLCdelta) fusion

Use

Tags

GFP

Expression

Yeast

Mutation

contains aa11-140 of PLCδ (including the PH domai…

Promoter

PRC1

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pRS426-CeLACE-1

Plasmid

#174523

Purpose

Express C.elegans LACE-1 in yeast cells

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

lace-1 (C30F12.2 Nematode)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

MET25

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Osh2-PH

Plasmid

#71373

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Use

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-Osh1-PH

Plasmid

#71372

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh1-PH

Use

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa267-388

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-PLCdelta-PH

Plasmid

#71375

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

PLCdelta-PH

Use

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain

Promoter

Available since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRS426 Gal TDP43

Plasmid

#27466

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
pRS426 TEF mCherry

Plasmid

#129433

Purpose

express mcherry

Depositor

Oskar Laur

Insert

mcherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS426-TUB1-internal6xHis

Plasmid

#60390

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-Num1-PH

Plasmid

#71374

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Use

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-COF1

Plasmid

#31844

Depositor

David Drubin

Article

Okreglak et al J Cell Biol. 2007 Sep 24;178(7):1251-64.

Insert

Cofilin (COF1 Budding Yeast)

Use

Tags

GFP (S65T)

Expression

Yeast

Mutation

Promoter

T7

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pRS426-PGAL7-A_GFP_TCYC1

Plasmid

#177162

Purpose

Expresses yeGFP under the control of a synthetic promoter (PGAL7-A)

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Synthetic promoter (PGAL7-A)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426-PGAL7-B_GFP_TCYC1

Plasmid

#177163

Purpose

Expresses yeGFP under the control of a synthetic promoter (PGAL7-B)

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Synthetic promoter (PGAL7-B)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS426-PGAL7-C_RFP_TCYC1

Plasmid

#177164

Purpose

Expresses yeRFP under the control of a synthetic promoter (PGAL7-C)

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Synthetic promoter (PGAL7-C)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS426-PGAL7-D_RFP_TCYC1

Plasmid

#177165

Purpose

Expresses yeRFP under the control of a synthetic promoter (PGAL7-D)

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

yeCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Synthetic promoter (PGAL7-D)

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge